验证数据展示
经过测试的应用
| Positive WB detected in | bEnd.3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31170-1-AP targets KLF2 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag33962 Product name: Recombinant human KLF2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 90-225 aa of NM_016270 Sequence: GLVSELLRPELDAPLGPALHGRFLLAPPGRLVKAEPPEADGGGGYGCAPGLTRGPRGLKREGAPGPAASCMRGPGGRPPPPPDTPPLSPDGPARLPAPGPRASFPPPFGGPGFGAPGPGLHYAPPAPPAFGLFDDA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Kruppel-like factor 2 (lung) |
| 别名 | Krueppel like factor 2, Krueppel-like factor 2, Kruppel like factor 2 (lung), LKLF, Lung krueppel like factor |
| 计算分子量 | 37KD |
| 观测分子量 | 37-40 kDa |
| GenBank蛋白编号 | NM_016270 |
| 基因名称 | KLF2 |
| Gene ID (NCBI) | 10365 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q9Y5W3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KLF2 (Kruppel-like Factor 2) belongs to zinc finger transcription factors, and it is implicated in a variety of biochemical processes, including lung development, embryonic erythropoiesis, epithelial integrity, T-cell viability, and adipogenesis (PMID: 17904406). KLF2 was identified as a negative regulator of T cell activation and adipogenesis. KLF2 also acts as a novel regulator of endothelial proinflammatory activation, inhibiting the inflammatory cytokine-mediated induction of adhesion molecule expression and immune cell adhesion (PMID:15718498). KLF2-null mice exhibit abnormal blood vessel formation, resulting in embryonic haemorrhage and death (PMID: 32132543). KLF2 can be detected 60 kDa by post-translationally modified (PMID: 36466816).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KLF2 antibody 31170-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
