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  • Featured Product
  • KD/KO Validated

KLF2 Polyclonal antibody

KLF2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 23384-1-AP

Print datasheet

Synonyms

KLF2, Krueppel like factor 2, Kruppel like factor 2 (lung), LKLF, Lung krueppel like factor

验证数据展示

  • WB analysis using 23384-1-AP

    WB analysis using 23384-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23384-1-AP (KLF2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHepG2 cells, bEnd.3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23384-1-AP targets KLF2 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA
Cited ApplicationsWB, IP
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen KLF2 fusion protein Ag20019 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Kruppel-like factor 2 (lung)
Synonyms KLF2, Krueppel like factor 2, Kruppel like factor 2 (lung), LKLF, Lung krueppel like factor
Calculated Molecular Weight 355 aa, 37 kDa
Observed Molecular Weight37-40 kDa, 60 kDa
GenBank Accession NumberBC071983
Gene Symbol KLF2
Gene ID (NCBI) 10365
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ9Y5W3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KLF2 (Kruppel-like Factor 2) belongs to zinc finger transcription factors, and it is implicated in a variety of biochemical processes, including lung development, embryonic erythropoiesis, epithelial integrity, T-cell viability, and adipogenesis (PMID: 17904406). KLF2 was identified as a negative regulator of T cell activation and adipogenesis. KLF2 also acts as a novel regulator of endothelial proinflammatory activation, inhibiting the inflammatory cytokine-mediated induction of adhesion molecule expression and immune cell adhesion (PMID:15718498). KLF2-null mice exhibit abnormal blood vessel formation, resulting in embryonic haemorrhage and death (PMID: 32132543). KLF2 can be detected 60 kDa by post-translationally modified (PMID: 36466816).

实验方案

Product Specific Protocols
WB protocol for KLF2 antibody 23384-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

J Thromb Haemost

IKKβ regulates endothelial thrombomodulin in a Klf2-dependent manner.

Authors - R Pathak
View Article