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验证数据展示

  • WB analysis using 19028-1-AP (same clone as 19028-1-PBS)

    WB analysis using 19028-1-AP (same clone as 19028-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19028-1-AP (KLC1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19028-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse brain using 19028-1-AP (same clone as 19028-1-PBS)

    WB analysis of mouse brain using 19028-1-AP (same clone as 19028-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 19028-1-AP (KLC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19028-1-PBS in a different storage buffer formulation.

  • IHC staining of human bowen disease using 19028-1-AP (same clone as 19028-1-PBS)

    IHC staining of human bowen disease using 19028-1-AP (same clone as 19028-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 19028-1-AP (KLC1 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19028-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 19028-1-AP (same clone as 19028-1-PBS)

    IHC staining of rat brain using 19028-1-AP (same clone as 19028-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 19028-1-AP (KLC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19028-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 19028-1-AP (same clone as 19028-1-PBS)

    IHC staining of mouse brain using 19028-1-AP (same clone as 19028-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19028-1-AP (KLC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19028-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 19028-1-AP (same clone as 19028-1-PBS)

    IF Staining of HeLa using 19028-1-AP (same clone as 19028-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using KLC1 antibody (19028-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 19028-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 19028-1-AP (same clone as 19028-1-PBS)

    IF Staining of HepG2 using 19028-1-AP (same clone as 19028-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using KLC1 antibody (19028-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 19028-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

KLC1 Polyclonal antibody, PBS Only

KLC1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 19028-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

KLC, KLC 1, KNS2, KNS2A

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

19028-1-PBS targets KLC1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag13570

Product name: Recombinant human KLC1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-290 aa of BC008881

Sequence: MYDNMSTMVYIKEDKLEKLTQDEIISKTKQVIQGLEALKNEHNSILQSLLETLKCLKKDDESNLVEEKSNMIRKSLEMLELGLSEAQVMMALSNHLNAVESEKQKLRAQVRRLCQENQWLRDELANTQQKLQKSEQSVAQLEEEKKHLEFMNQLKKYDDDISPSEDKDTDSTKEPLDDLFPNDEDDPGQGIQQQHSSAAAAAQQGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHDHPDVATMLNILALVYRDQNKYKDAANLLNDALAIREKTLG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kinesin light chain 1
别名 KLC, KLC 1, KNS2, KNS2A
计算分子量 65 kDa
观测分子量 61-70 kDa
GenBank蛋白编号BC008881
基因名称 KLC1
Gene ID (NCBI) 3831
RRIDAB_10641041
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ07866
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. Kinesin is tetrameric proteins containing two heavy (alpha) chains and two light (beta) chains. The alpha chains provide the tubulin binding site and the ATPase domains, whereas the beta chains are responsible for the specific attachment of the organelle to be moved by the kinesin tetramer (PubMed:21385839).

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