• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 19028-1-AP

    WB analysis using 19028-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19028-1-AP (KLC1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 19028-1-AP

    IHC staining of human brain using 19028-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 19028-1-AP (KLC1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 19028-1-AP

    IHC staining of human brain using 19028-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 19028-1-AP (KLC1 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HeLa using 19028-1-AP

    IF Staining of HeLa using 19028-1-AP

    Immunofluorescent analysis of Hela cells, using KLC1 antibody 19028-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • WB analysis of mouse brain using 19028-1-AP

    WB analysis of mouse brain using 19028-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 19028-1-AP (KLC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human bowen disease using 19028-1-AP

    IHC staining of human bowen disease using 19028-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 19028-1-AP (KLC1 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

KLC1 Polyclonal antibody

KLC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 19028-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

KLC, KLC 1, KNS2, KNS2A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inSH-SY5Y cells, COLO 320 cells, HepG2 cells, mouse brain tissue, SK-N-SH cells
Positive IHC detected inhuman brain tissue, human bowen disease
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells, HepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:100-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19028-1-AP targets KLC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 KLC1 fusion protein Ag13570 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kinesin light chain 1
别名 KLC, KLC 1, KNS2, KNS2A
计算分子量 65 kDa
观测分子量 61-70 kDa
GenBank蛋白编号BC008881
基因名称 KLC1
Gene ID (NCBI) 3831
RRIDAB_10641041
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ07866
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. Kinesin is tetrameric proteins containing two heavy (alpha) chains and two light (beta) chains. The alpha chains provide the tubulin binding site and the ATPase domains, whereas the beta chains are responsible for the specific attachment of the organelle to be moved by the kinesin tetramer (PubMed:21385839).

实验方案

Product Specific Protocols
WB protocol for KLC1 antibody 19028-1-APDownload protocol
IHC protocol for KLC1 antibody 19028-1-APDownload protocol
IF protocol for KLC1 antibody 19028-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

FASEB J

Kinesin-dependent transport of keratin filaments: a unified mechanism for intermediate filament transport.

Authors - Amélie Robert
  • KO Validated
View Article
humanWB,IF

Dev Neurobiol

Forebrain commissure formation in zebrafish embryo requires the binding of KLC1 to CRMP2

Authors - Simo Li
View Article