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验证数据展示

  • WB analysis using 18375-1-AP (same clone as 18375-1-PBS)

    WB analysis using 18375-1-AP (same clone as 18375-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18375-1-AP (KISS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18375-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 18375-1-AP (same clone as 18375-1-PBS)

    IHC staining of human placenta using 18375-1-AP (same clone as 18375-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18375-1-AP (KISS1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18375-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KISS1 antibody included
  • IHC staining of human liver cancer using 18375-1-AP (same clone as 18375-1-PBS)

    IHC staining of human liver cancer using 18375-1-AP (same clone as 18375-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18375-1-AP (KISS1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 18375-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KISS1 antibody included
  • IF Staining of NIH/3T3 using 18375-1-PBS

    IF Staining of NIH/3T3 using 18375-1-PBS

    Immunofluorescent analysis of NIH/3T3 cells using 18375-1-AP (KISS1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO Validated

KISS1 Polyclonal antibody, PBS Only

KISS1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18375-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

KiSS 1, KiSS 1 metastasis suppressor, Kisspeptin 1, Kisspeptin-1, Metastasis suppressor KiSS 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy KISS1 Ready-To-Use IHC Kit. KISS1 primary antibody included.

产品信息

18375-1-PBS targets KISS1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag13240

Product name: Recombinant human KISS1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-138 aa of BC022819

Sequence: MNSLVSWQLLLFLCATHFGEPLEKVASVGNSRPTGQQLESLGLLAPGEQSLPCTERKPAATARLSRRGTSLSPPPESSGSPQQPGLSAPHSRQIPAPQGAVLVQREKDLPNYNWNSFGLRFGKREAAPGNHGRSAGRG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 KiSS-1 metastasis-suppressor
别名 KiSS 1, KiSS 1 metastasis suppressor, Kisspeptin 1, Kisspeptin-1, Metastasis suppressor KiSS 1
计算分子量 15 kDa
观测分子量15 kDa
GenBank蛋白编号BC022819
基因名称 KISS1
Gene ID (NCBI) 3814
RRIDAB_11182705
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15726
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

KISS1, also known as kisspeptin, is a metastasis suppressor protein that suppresses metastases of human melanomas and breast carcinomas without affecting tumorigenicity. It may regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. A C-terminally amidated peptide of KISS1, metastin (kisspeptin-54 or kp-54), functions as the endogenous ligand of the G-protein coupled receptor GPR54 (PMID: 11457843; 11385580). KISS1-GPR54 signaling is involved in the regulation of reproduction, playing an important role in initiating secretion of gonadotropin-releasing hormone (GnRH) at puberty (PMID: 18753370).

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