验证数据展示
经过测试的应用
| Positive WB detected in | human placenta tissue |
| Positive IHC detected in | human placenta tissue, human liver cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IHC | See 3 publications below |
产品信息
18375-1-AP targets KISS1 in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human, rat |
| 免疫原 |
CatNo: Ag13240 Product name: Recombinant human KISS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-138 aa of BC022819 Sequence: MNSLVSWQLLLFLCATHFGEPLEKVASVGNSRPTGQQLESLGLLAPGEQSLPCTERKPAATARLSRRGTSLSPPPESSGSPQQPGLSAPHSRQIPAPQGAVLVQREKDLPNYNWNSFGLRFGKREAAPGNHGRSAGRG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | KiSS-1 metastasis-suppressor |
| 别名 | KiSS 1, KiSS 1 metastasis suppressor, Kisspeptin 1, Kisspeptin-1, Metastasis suppressor KiSS 1 |
| 计算分子量 | 15 kDa |
| 观测分子量 | 15 kDa |
| GenBank蛋白编号 | BC022819 |
| 基因名称 | KISS1 |
| Gene ID (NCBI) | 3814 |
| RRID | AB_11182705 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15726 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KISS1, also known as kisspeptin, is a metastasis suppressor protein that suppresses metastases of human melanomas and breast carcinomas without affecting tumorigenicity. It may regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. A C-terminally amidated peptide of KISS1, metastin (kisspeptin-54 or kp-54), functions as the endogenous ligand of the G-protein coupled receptor GPR54 (PMID: 11457843; 11385580). KISS1-GPR54 signaling is involved in the regulation of reproduction, playing an important role in initiating secretion of gonadotropin-releasing hormone (GnRH) at puberty (PMID: 18753370).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for KISS1 antibody 18375-1-AP | Download protocol |
| WB protocol for KISS1 antibody 18375-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Oncol Ryanodine receptor 2 promotes colorectal cancer metastasis by the ROS/BACH1 axis | ||
Int J Cancer Long noncoding RNA TC0101441 induces epithelial-mesenchymal transition in epithelial ovarian cancer metastasis by downregulating KiSS1.
| ||
Histochem Cell Biol Functional analysis of miR-767-5p during the progression of hepatocellular carcinoma and the clinical relevance of its dysregulation. | ||
Oncol Lett Analysis of the association of the expression of KiSS-1 in colorectal cancer tissues with the pathology and prognosis. | ||
Cells Kisspeptin Alleviates Human Hepatic Fibrogenesis by Inhibiting TGFβ Signaling in Hepatic Stellate Cells | ||
Cell Signal Overexpression of Rap1B alleviate central precocious puberty and neurodevelopmental damage |
