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KIR3DL1 Polyclonal antibody

KIR3DL1 Polyclonal Antibody for FC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, WB,ELISA

Conjugate

Unconjugated

Cat No : 13335-1-AP

Print datasheet

Synonyms

CD158E, CD158E1, KIR, KIR3DL1, MHC class I NK cell receptor, NKAT 3, NKAT3, NKB1, NKB1B, p70 NK receptor CL 2/CL 11

验证数据展示

  • WB analysis of human liver using 13335-1-AP

    WB analysis of human liver using 13335-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 13335-1-AP (KIR3DL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • FC experiment of Jurkat using 13335-1-AP

    FC experiment of Jurkat using 13335-1-AP

    1X10^6 Jurkat cells were stained with 0.2ug KIR3DL1 antibody (13335-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

经过测试的应用

Positive WB detected inhuman liver tissue
Positive FC detected inJurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13335-1-AP targets KIR3DL1 in WB, FC, ELISA applications and shows reactivity with human samples.

Tested Applications FC, WB,ELISA
Tested Reactivity human
Immunogen KIR3DL1 fusion protein Ag4152 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name killer cell immunoglobulin-like receptor, three domains, long cytoplasmic tail, 1
Synonyms CD158E, CD158E1, KIR, KIR3DL1, MHC class I NK cell receptor, NKAT 3, NKAT3, NKB1, NKB1B, p70 NK receptor CL 2/CL 11
Calculated Molecular Weight 444 aa, 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC028206
Gene Symbol KIR3DL1
Gene ID (NCBI) 3811
RRIDAB_2234189
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP43629
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR3DL1 is a receptor on natural killer (NK) cells for HLA Bw4 allele. It inhibits the activity of NK cells thus preventing cell lysis. KIR3DL1 and KIR3DS1 are highly homologous. This antibody is raised against 22-344 amino acids of human KIR3DL1 and recognizes both KIR3DL1 (49 kDa) and KIR3DS1 (43 kDa).

实验方案

Product Specific Protocols
WB protocol for KIR3DL1 antibody 13335-1-APDownload protocol
FC protocol for KIR3DL1 antibody 13335-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Clin Exp Immunol

Autoantibodies to Killer Cell Immunoglobulin-Like Receptor 3DL1 in Patients with Systemic Lupus Erythematosus.

Authors - Akihiro Doi
View Article