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验证数据展示

  • WB analysis of HL-60 using 21152-1-AP

    WB analysis of HL-60 using 21152-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 21152-1-AP (KIR2DS4/CD158i antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 21152-1-AP

    IF Staining of HepG2 using 21152-1-AP

    Immunofluorescent analysis of HepG2 cells, using KIR2DS4/CD158i antibody 21152-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

KIR2DS4/CD158i Polyclonal antibody

KIR2DS4/CD158i Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 21152-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

KIR2DS4, CD158 antigen-like family member I, CD158I, Killer cell immunoglobulin-like receptor 2DS4, KIR1D

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHL-60 cells, Jurkat cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21152-1-AP targets KIR2DS4/CD158i in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag15711

Product name: Recombinant human KIR2DS4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 21-304 aa of BC096694

Sequence: PQEGVHRKPSFLALPGHLVKSEETVILQCWSDVMFEHFLLHREGKFNNTLHLIGEHHDGVSKANFSIGPMMPVLAGTYRCYGSVPHSPYQLSAPSDPLDMVIIGLYEKPSLSAQPGPTVQAGENVTLSCSSRSSYDMYHLSREGEAHERRLPAVRSINGTFQADFPLGPATHGGTYRCFGSFRDAPYEWSNSSDPLLVSVTGNPSNSWPSPTEPSSKTGNPRHLHVLIGTSVVKIPFTILLFFLLHRWCSDKKNAAVMDQEPAGNRTVNSEDSDEQDHQEVSYA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail, 4
别名 KIR2DS4, CD158 antigen-like family member I, CD158I, Killer cell immunoglobulin-like receptor 2DS4, KIR1D
计算分子量 304 aa, 34 kDa
观测分子量 33 kDa
GenBank蛋白编号BC096694
基因名称 KIR2DS4
Gene ID (NCBI) 3809
RRIDAB_10732956
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP43632
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIR2DS4 (also known as CD158i) is a member of the killer cell immunoglobulin-like receptor (KIR) family. KIRs are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. KIRs are mainly involved in inhibiting NK killing (inhibitory KIRs) via interaction with MHC class I molecules. KIR2DS4 has been reported to be an activating KIR and recognize the HLA-Cw4 protein.

实验方案

Product Specific Protocols
WB protocol for KIR2DS4/CD158i antibody 21152-1-APDownload protocol
IF protocol for KIR2DS4/CD158i antibody 21152-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols