- Featured Product
- KD/KO Validated
KIFC1 Polyclonal antibody
KIFC1 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IHC, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | rat testis tissue, HeLa cells, mouse testis tissue, NIH/3T3 cells |
| Positive IHC detected in | human placenta tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | Hela cells, HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of 20790-1-AP is KIFC1 Fusion Protein expressed in E. coli.
| Tested Applications | IF/ICC, IHC, WB, ELISA |
| Cited Applications | WB, IF, IHC, Peptide array |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | KIFC1 fusion protein Ag14492 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | kinesin family member C1 |
| Synonyms | HSET, KIFC1, kinesin family member C1, Kinesin like protein 2, Kinesin like protein KIFC1, Kinesin related protein HSET, KNSL2 |
| Calculated Molecular Weight | 673 aa, 74 kDa |
| Observed Molecular Weight | 55-70 kDa |
| GenBank Accession Number | BC000712 |
| Gene Symbol | KIFC1 |
| Gene ID (NCBI) | 3833 |
| RRID | AB_10755286 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BW19 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KIFC1 is a molecular motor protein belonging to the kinesin-14 family that has motor domains at the C-terminus. KIFC1 is widely distributed, with highest expression in testis and participating in acrosome biogenesis and vesicle transport. It is also abundant in ovary, spleen, and liver. KIFC1 proteins are generally localized in the perinuclear region, and have been found to be involved chromosome alignment, congression and intracellular DNA transport. This antibody was raised against the C-terminal region of human KIFC1 protein, and recognizes 55-70 kDa of endogenous KIFC1 protein in multiple tissue lysates. (12826589, 15236353, 23543461)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KIFC1 antibody 20790-1-AP | Download protocol |
| IHC protocol for KIFC1 antibody 20790-1-AP | Download protocol |
| IF protocol for KIFC1 antibody 20790-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Science Mechanism of spindle pole organization and instability in human oocytes.
| ||
Dev Cell Direct Microtubule-Binding by Myosin-10 Orients Centrosomes toward Retraction Fibers and Subcortical Actin Clouds. | ||
Cell Mol Life Sci Proteomic interaction profiling reveals KIFC1 as a factor involved in early targeting of F508del-CFTR to degradation.
| ||
FASEB J Export of membrane proteins from the Golgi complex to the primary cilium requires the kinesin motor, KIFC1. | ||
Front Mol Biosci Prognostic Value and Immunological Role of KIFC1 in Hepatocellular Carcinoma. |
