验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, A549 cells, HeLa cells |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12760-1-AP targets KIF1C in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag3500 Product name: Recombinant human KIF1C protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-273 aa of BC034993 Sequence: MAGASVKVAVRVRPFNARETSQDAKCVVSMQGNTTSIINPKQSKDAPKSFTFDYSYWSHTSTEDPQFASQQQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGRQEPGQQGIVPQLCEDLFSRVSENQSAQLSYSVEVSYMEIYCERVRDLLNPKSRGSLRVREHPILGPYVQDLSKLAVTSYADIADLMDCGNKARTVAATNMNETSSRSHAVFTIVFTQRCHDQLTGLDSEKVSKISLVDLAGSERADSSGARGMRLKEGANINK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | kinesin family member 1C |
| 别名 | KIAA0706, KIF1C, kinesin family member 1C, Kinesin like protein KIF1C, LTXS1 |
| 计算分子量 | 1103 aa, 123 kDa |
| 观测分子量 | 135-140 kDa |
| GenBank蛋白编号 | BC034993 |
| 基因名称 | KIF1C |
| Gene ID (NCBI) | 10749 |
| RRID | AB_2131422 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O43896 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for KIF1C antibody 12760-1-AP | Download protocol |
| IP protocol for KIF1C antibody 12760-1-AP | Download protocol |
| WB protocol for KIF1C antibody 12760-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Muscleblind-like proteins use modular domains to localize RNAs by riding kinesins and docking to membranes | ||
Cell Rep A KIF1C-CNBP motor-adaptor complex for trafficking mRNAs to cell protrusions
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