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  • Featured Product
  • KD/KO Validated

KIF1C Polyclonal antibody

KIF1C Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 12760-1-AP

Print datasheet

Synonyms

KIAA0706, KIF1C, kinesin family member 1C, Kinesin like protein KIF1C, LTXS1

验证数据展示

  • WB analysis of mouse heart using 12760-1-AP

    WB analysis of mouse heart using 12760-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 12760-1-AP (KIF1C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 12760-1-AP

    IHC staining of human gliomas using 12760-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12760-1-AP (KIF1C antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human gliomas using 12760-1-AP

    IHC staining of human gliomas using 12760-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12760-1-AP (KIF1C antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of mouse heart using 12760-1-AP

    IP experiment of mouse heart using 12760-1-AP

    IP result of anti-KIF1C (IP:12760-1-AP, 5ug; Detection:12760-1-AP 1:300) with mouse heart tissue lysate 4000ug.

  • WB analysis using 12760-1-AP

    WB analysis using 12760-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12760-1-AP (KIF1C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse heart tissue, A549 cells, HeLa cells
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12760-1-AP targets KIF1C in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsWB, IP, IF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen KIF1C fusion protein Ag3500 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kinesin family member 1C
Synonyms KIAA0706, KIF1C, kinesin family member 1C, Kinesin like protein KIF1C, LTXS1
Calculated Molecular Weight 1103 aa, 123 kDa
Observed Molecular Weight 135-140 kDa
GenBank Accession NumberBC034993
Gene Symbol KIF1C
Gene ID (NCBI) 10749
RRIDAB_2131422
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43896
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KIF1C antibody 12760-1-APDownload protocol
IHC protocol for KIF1C antibody 12760-1-APDownload protocol
IP protocol for KIF1C antibody 12760-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

RNA

The kinesin KIF1C transports APC-dependent mRNAs to cell protrusions.

Authors - Xavier Pichon
  • KD Validated
View Article
mouseWB,IF,IP

Nat Commun

Muscleblind-like proteins use modular domains to localize RNAs by riding kinesins and docking to membranes

Authors - Ryan P Hildebrandt
View Article