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验证数据展示

  • WB analysis using 27666-1-AP

    WB analysis using 27666-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27666-1-AP (EPG5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 27666-1-AP

    IHC staining of human kidney using 27666-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27666-1-AP (KIAA1632 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

  • IHC staining of human kidney using 27666-1-AP

    IHC staining of human kidney using 27666-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27666-1-AP (KIAA1632 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

  • IHC staining of human lung cancer using 27666-1-AP

    IHC staining of human lung cancer using 27666-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27666-1-AP (EPG5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

EPG5 Polyclonal antibody

EPG5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27666-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

HEEW1, KIAA1632, UPF0493 protein KIAA1632

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman kidney tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27666-1-AP targets EPG5 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag24801

Product name: Recombinant human KIAA1632 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 2112-2462 aa of BC130614

Sequence: ETRSMIVCLLFMMILLAKEVQLVDQTDSPLLSLLGQTSSLSWHLVDIVSYQSVLSYFSSHYPPSIILAKESYAELIMKLLKVSAGLSIPTDSQKHLDAVPKCQAFTHQMVQFLSTLEQNGKITLAVLEQEMSKLLDDIIVFNPPDMDSQTRHMALSSLFMEVLMMMNNATIPTAEFLRGSIRTWIGQKMHGLVVLPLLTAACQSLASVRHMAETTEACITAYFKESPLNQNSGWGPILVSLQVPELTMEEFLQECLTLGSYLTLYVYLLQCLNSEQTLRNEMKVLLILSKWLEQVYPSSVEEEAKLFLWWHQVLQLSLIQTEQNDSVLTESVIRILLLVQSRQNLVAEERL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 KIAA1632
别名 HEEW1, KIAA1632, UPF0493 protein KIAA1632
计算分子量 2579 aa, 292 kDa
观测分子量270-290 kDa
GenBank蛋白编号BC130614
基因名称 EPG5
Gene ID (NCBI) 57724
RRIDAB_3085981
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HCE0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EPG5, a metazoan-specific autophagy protein, regulates autophagy by promoting fusion of autophagosomes with lysosomes and/or late endosomes. EPG5 regulates the stability and the assembly of STX17-SNAP29-VAMP8 trans-SNARE complexes. Loss of function of EPG5 causes non-specific fusion of autophagosomes with other endocytic vesicles, resulting in formation of non-degradative enlarged vesicles. EPG5 expression in human pluripotent stem cells like ESC and iPSC is higher than that of human somatic cells.(PMID: 30931944, PMID: 27588602)

实验方案

Product Specific Protocols
IHC protocol for EPG5 antibody 27666-1-APDownload protocol
WB protocol for EPG5 antibody 27666-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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