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验证数据展示

  • WB analysis using 18993-1-AP

    WB analysis using 18993-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18993-1-AP (KIAA1468 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 18993-1-AP

    IP experiment of mouse brain using 18993-1-AP

    IP result of anti-KIAA1468 (IP:18993-1-AP, 4ug; Detection:18993-1-AP 1:500) with mouse brain tissue lysate 1280 ug.

  • IHC staining of mouse kidney using 18993-1-AP

    IHC staining of mouse kidney using 18993-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18993-1-AP (KIAA1468 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KIAA1468 antibody included
  • IHC staining of mouse kidney using 18993-1-AP

    IHC staining of mouse kidney using 18993-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18993-1-AP (KIAA1468 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KIAA1468 antibody included

KIAA1468 Polyclonal antibody

KIAA1468 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 18993-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

RELCH, HsT3308, HsT885, LisH domain and HEAT repeat-containing protein KIAA1468, RAB11 binding and LisH domain, coiled-coil and HEAT repeat-containing

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy® KIAA1468 Ready-To-Use IHC Kit. KIAA1468 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18993-1-AP targets KIAA1468 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 KIAA1468
别名 RELCH, HsT3308, HsT885, LisH domain and HEAT repeat-containing protein KIAA1468, RAB11 binding and LisH domain, coiled-coil and HEAT repeat-containing
计算分子量 139 kDa
观测分子量 100-135 kDa
GenBank蛋白编号NM_020854
基因名称 KIAA1468
Gene ID (NCBI) 57614
RRIDAB_10598164
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9P260
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RELCH/KIAA1468, which is a newly identified Rab11 effector protein, tethers the recycling endosomes (REs) and trans-Golgi network (TGN) membranes by binding TGN-localized oxysterol-binding protein (OSBP) and promotes OSBP-dependent nonvesicular cholesterol transport from REs to the TGN.

实验方案

Product Specific Protocols
IHC protocol for KIAA1468 antibody 18993-1-APDownload protocol
IP protocol for KIAA1468 antibody 18993-1-APDownload protocol
WB protocol for KIAA1468 antibody 18993-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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