验证数据展示
经过测试的应用
Positive FC (Intra) detected in | MCF-7 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IF | See 1 publications below |
产品信息
CL488-68235 targets KIAA1429 in IF, FC (Intra) applications and shows reactivity with human, mouse samples.
经测试应用 | FC (Intra) Application Description |
文献引用应用 | IF |
经测试反应性 | human, mouse |
文献引用反应性 | human |
免疫原 | KIAA1429 fusion protein Ag22449 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | KIAA1429 |
别名 | VIRMA, 3F1A4, MSTP054, virilizer |
计算分子量 | 1812 aa, 202 kDa |
观测分子量 | 200-210 kDa |
GenBank蛋白编号 | BC113380 |
基因名称 | KIAA1429 |
Gene ID (NCBI) | 25962 |
RRID | AB_3084451 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | Q69YN4 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
VIRMA (KIAA1429) is a key component of m6A methyltransferase (writer) complex that include METTL3, METTL14, WTAP, VIRMA, HAKAI, ZC3H13, and RBM15. VIRMA mediates methylation in the 3'UTR and around the stop codon, thus affecting alternative polyadenylation. VIRMA has been reported to act as an oncogenic factor in breast cancer and liver cancer. KIAA1429 exhibits differential nuclear and cytoplasmic distribution in colorectal cancer samples, and high cytoplasmic expression of KIAA1429 is associated with poor response to chemotherapy. BRAF-mediated phosphorylation of KIAA1429 facilitates its aggregation in the cytoplasm. Furthermore, cytoplasmic KIAA1429 promotes WNT pathway activation by binding and stabilizing FZD7, thereby further enhancing cancer stemness and oxaliplatin resistance (PMID: 40611274).
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 488 KIAA1429 antibody CL488-68235 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |