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验证数据展示

  • FC experiment of MCF-7 using CL488-68235

    FC experiment of MCF-7 using CL488-68235

    1X10^6 MCF-7 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human KIAA1429 (CL488-68235, Clone:3F1A4) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated KIAA1429 Monoclonal antibody

KIAA1429 Monoclonal Antibody for FC (Intra)
Cat No. CL488-68235

产品说明书

CloneNo. 3F1A4
引用文献(1)

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

FC (Intra) and More (1)

别名

VIRMA, 3F1A4, MSTP054, virilizer

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy VIRMA/KIAA1429 Ready-To-Use IHC Kit. VIRMA/KIAA1429 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68235-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

CL488-68235 targets KIAA1429 in IF, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 FC (Intra) Application Description
文献引用应用IF
经测试反应性 human, mouse
文献引用反应性human
免疫原 KIAA1429 fusion protein Ag22449 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 KIAA1429
别名 VIRMA, 3F1A4, MSTP054, virilizer
计算分子量 1812 aa, 202 kDa
观测分子量200-210 kDa
GenBank蛋白编号BC113380
基因名称 KIAA1429
Gene ID (NCBI) 25962
RRIDAB_3084451
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ69YN4
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VIRMA (KIAA1429) is a key component of m6A methyltransferase (writer) complex that include METTL3, METTL14, WTAP, VIRMA, HAKAI, ZC3H13, and RBM15. VIRMA mediates methylation in the 3'UTR and around the stop codon, thus affecting alternative polyadenylation. VIRMA has been reported to act as an oncogenic factor in breast cancer and liver cancer. KIAA1429 exhibits differential nuclear and cytoplasmic distribution in colorectal cancer samples, and high cytoplasmic expression of KIAA1429 is associated with poor response to chemotherapy. BRAF-mediated phosphorylation of KIAA1429 facilitates its aggregation in the cytoplasm. Furthermore, cytoplasmic KIAA1429 promotes WNT pathway activation by binding and stabilizing FZD7, thereby further enhancing cancer stemness and oxaliplatin resistance (PMID: 40611274).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 KIAA1429 antibody CL488-68235Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

J Exp Clin Cancer Res

Phosphorylation of KIAA1429 promotes oxaliplatin resistance through activating the FZD7-Wnt signaling in BRAFV600E-mutated colorectal cancer

Authors - Taixuan Wan
View Article