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KIAA1217 Polyclonal antibody

KIAA1217 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 24880-1-AP

Print datasheet

Synonyms

KIAA1217, Sickle tail protein homolog, SKT

验证数据展示

  • WB analysis using 24880-1-AP

    WB analysis using 24880-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24880-1-AP (KIAA1217 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis using 24880-1-AP

    WB analysis using 24880-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24880-1-AP (KIAA1217 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, rat colon tissue, mouse colon tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24880-1-AP targets KIAA1217 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen KIAA1217 fusion protein Ag19367 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name KIAA1217
Synonyms KIAA1217, Sickle tail protein homolog, SKT
Calculated Molecular Weight 1943 aa, 214 kDa
Observed Molecular Weight100-110 kDa, 140-160 kDa, 210 kDa
GenBank Accession NumberBC018764
Gene Symbol KIAA1217
Gene ID (NCBI) 56243
RRIDAB_2879775
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5T5P2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIAA1217, sickle tail protein homolog, is required for normal development of intervertebral disks. It promotes epithelial-mesenchymal transition and hepatocellular carcinoma metastasis by interacting with and activating STAT3, indicating its potential as a therapeutic target for curbing HCC metastasis (PMID: 35008530). It is also a novel candidate gene associated with isolated and syndromic vertebral malformations (PMID: 32369272). KIAA1217 has 9 alternatively spliced isoforms of 100-110 kDa, 140-160 kDa, 210 kDa.

实验方案

Product Specific Protocols
WB protocol for KIAA1217 antibody 24880-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols