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验证数据展示

  • WB analysis using 24880-1-AP

    WB analysis using 24880-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24880-1-AP (KIAA1217 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis using 24880-1-AP

    WB analysis using 24880-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24880-1-AP (KIAA1217 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

KIAA1217 Polyclonal antibody

KIAA1217 Polyclonal Antibody for WB, ELISA
Cat No. 24880-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

KIAA1217, Sickle tail protein homolog, SKT

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, rat colon tissue, mouse colon tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24880-1-AP targets KIAA1217 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag19367

Product name: Recombinant human KIAA1217 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 534-852 aa of BC018764

Sequence: PAQNLPHVASSPAVPQEATSTLQMSQAPQSPQIPMNGSAMQSLFIEEIHSVSAKNRAVSIEKAEKKWEEKRQNLDHYNGKEFEKLLEEAQANIMKSIPNLEMPPATGPLPRGDAPVDKVELSEDSPNSEQDLEKLGGKSPPPPPPPPRRSYLPGSGLTTTRSGDVVYTGRKENITAKASSEDAGPSPQTRATKYPAEEPASAWTPSPPPVTTSSSKDEEEEEEEGDKIMAELQAFQKCSFMDVNSNSHAEPSRADSHVKDTRSGATVPPKEKKNLEFFHEDVRKSDVEYENGPQMEFQKGSSGAPQTSRMPVPMSAKNR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 KIAA1217
别名 KIAA1217, Sickle tail protein homolog, SKT
计算分子量 1943 aa, 214 kDa
观测分子量100-110 kDa, 140-160 kDa, 210 kDa
GenBank蛋白编号BC018764
基因名称 KIAA1217
Gene ID (NCBI) 56243
RRIDAB_2879775
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5T5P2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIAA1217, sickle tail protein homolog, is required for normal development of intervertebral disks. It promotes epithelial-mesenchymal transition and hepatocellular carcinoma metastasis by interacting with and activating STAT3, indicating its potential as a therapeutic target for curbing HCC metastasis (PMID: 35008530). It is also a novel candidate gene associated with isolated and syndromic vertebral malformations (PMID: 32369272). KIAA1217 has 9 alternatively spliced isoforms of 100-110 kDa, 140-160 kDa, 210 kDa.

实验方案

Product Specific Protocols
WB protocol for KIAA1217 antibody 24880-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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