验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HeLa cells |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
51101-1-AP targets KIAA1033 in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | KIAA1033 |
| 别名 | WASHC4, WASH complex subunit SWIP, WASH complex subunit 7, WASH complex subunit 4, SWIP |
| 计算分子量 | 136.4 kDa |
| 观测分子量 | 136 kDa |
| GenBank蛋白编号 | BC104994 |
| 基因名称 | KIAA1033 |
| Gene ID (NCBI) | 23325 |
| RRID | AB_2881248 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q2M389 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KIAA1033, also named as WASH-interacting protein and SWIP, is a component of WASH complex. The WASH complex plays a key role in the fission of tubules that serve as transport intermediates during endosome sorting. KIAA1033 has two isoforms with MW 100 kDa and 136 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KIAA1033 antibody 51101-1-AP | Download protocol |
| IHC protocol for KIAA1033 antibody 51101-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Pigment Cell Melanoma Res Loss of strumpellin in the melanocytic lineage impairs the WASH Complex but does not affect coat colour. | ||
Autophagy RAB21 controls autophagy and cellular energy homeostasis by regulating retromer-mediated recycling of SLC2A1/GLUT1 | ||
Immunology SNX17 Regulates Antigen Internalisation and Phagosomal Maturation by Dendritic Cells |
