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验证数据展示

  • WB analysis using 21016-1-AP (same clone as 21016-1-PBS)

    WB analysis using 21016-1-AP (same clone as 21016-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21016-1-AP (KIAA0319L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21016-1-PBS in a different storage buffer formulation.

  • WB analysis using 21016-1-AP (same clone as 21016-1-PBS)

    WB analysis using 21016-1-AP (same clone as 21016-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21016-1-AP (KIAA0319L antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21016-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 21016-1-AP (same clone as 21016-1-PBS)

    IHC staining of rat brain using 21016-1-AP (same clone as 21016-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21016-1-AP (KIAA0319L antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21016-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 21016-1-AP (same clone as 21016-1-PBS)

    IHC staining of rat brain using 21016-1-AP (same clone as 21016-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21016-1-AP (KIAA0319L antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21016-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 21016-1-AP (same clone as 21016-1-PBS)

    IHC staining of mouse brain using 21016-1-AP (same clone as 21016-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21016-1-AP (KIAA0319L antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21016-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 21016-1-AP (same clone as 21016-1-PBS)

    IHC staining of mouse brain using 21016-1-AP (same clone as 21016-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21016-1-AP (KIAA0319L antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21016-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

KIAA0319L Polyclonal antibody, PBS Only

KIAA0319L Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 21016-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

AAVR, Adeno-associated virus receptor, Dyslexia-associated protein KIAA0319-like protein, KIAA0319 like, KIAA1837

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

21016-1-PBS targets KIAA0319L in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag15263

Product name: Recombinant human KIAA0319L protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 233-582 aa of BC031672

Sequence: ATAQVTVIVQPENNKPPQADAGPDKELTLPVDSTTLDGSKSSDDQKIISYLWEKTQGPDGVQLENANSSVATVTGLQVGTYVFTLTVKDERNLQSQSSVNVIVKEEINKPPIAKITGNVVITLPTSTAELDGSKSSDDKGIVSYLWTRDEGSPAAGEVLNHSDHHPILFLSNLVEGTYTFHLKVTDAKGESDTDRTTVEVKPDPRKNNLVEIILDINVSQLTERLKGMFIRQIGVLLGVLDSDIIVQKIQPYTEQSTKMVFFVQNEPPHQIFKGHEVAAMLKSELRKQKADFLIFRALEVNTVTCQLNCSDHGHCDSFTKRCICDPFWMENFIKVQLRDGDSNCEWSVLY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 KIAA0319-like
别名 AAVR, Adeno-associated virus receptor, Dyslexia-associated protein KIAA0319-like protein, KIAA0319 like, KIAA1837
计算分子量 1049 aa, 116 kDa
观测分子量 ~150 kDa
GenBank蛋白编号BC031672
基因名称 KIAA0319L
Gene ID (NCBI) 79932
RRIDAB_10733523
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IZA0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

KIAA0319L (also known as AAVR) is a type-I transmembrane protein that acts as a receptor for adeno-associated virus (AAV) and is involved in adeno-associated virus infection through endocytosis system (PMID: 26814968). It has been shown that KIAA0319L is highly-glycosylated and can localise to the plasma membrane, endosomes, Golgi and trans-Golgi network (TGN) (PMID: 29991750). KIAA0319L gene has been associated with susceptibility to developmental dyslexia or reading disabilities (PMID: 19085271). The predicted molecular mass of KIAA0319L is 116 kDa, but western blot detected protein at 150 kDa in lysate from HeLa cells, likely due to glycosidic linkages (PMID: 28679762; 30962536).

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