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KIAA0319L Polyclonal antibody
KIAA0319L Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IHC, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, MCF-7 cells |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of 21016-1-AP is KIAA0319L Fusion Protein expressed in E. coli.
| Tested Applications | IHC, WB, ELISA |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | KIAA0319L fusion protein Ag15263 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | KIAA0319-like |
| Synonyms | AAVR, Adeno-associated virus receptor, KIAA0319 like, KIAA0319L, KIAA1837, PKD1 like, PP791, RP4 765A10.3 |
| Calculated Molecular Weight | 1049 aa, 116 kDa |
| Observed Molecular Weight | 150 kDa |
| GenBank Accession Number | BC031672 |
| Gene Symbol | KIAA0319L |
| Gene ID (NCBI) | 79932 |
| RRID | AB_10733523 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IZA0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KIAA0319L (also known as AAVR) is a type-I transmembrane protein that acts as a receptor for adeno-associated virus (AAV) and is involved in adeno-associated virus infection through endocytosis system (PMID: 26814968). It has been shown that KIAA0319L is highly-glycosylated and can localise to the plasma membrane, endosomes, Golgi and trans-Golgi network (TGN) (PMID: 29991750). KIAA0319L gene has been associated with susceptibility to developmental dyslexia or reading disabilities (PMID: 19085271). The predicted molecular mass of KIAA0319L is 116 kDa, but western blot detected protein at 150 kDa in lysate from HeLa cells, likely due to glycosidic linkages (PMID: 28679762; 30962536).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KIAA0319L antibody 21016-1-AP | Download protocol |
| IHC protocol for KIAA0319L antibody 21016-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Methods Hardwiring tissue-specific AAV transduction in mice through engineered receptor expression | ||
Sci Adv Enhancement of liver-directed transgene expression at initial and repeat doses of AAV vectors admixed with ImmTOR nanoparticles.
| ||
Sci Rep AU040320 deficiency leads to disruption of acrosome biogenesis and infertility in homozygous mutant mice. | ||
Sci Rep Correction of LAMP3-associated salivary gland hypofunction by aquaporin gene therapy |