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验证数据展示

  • IF Staining of HeLa using 82931-5-RR (same clone as 82931-5-PBS)

    IF Staining of HeLa using 82931-5-RR (same clone as 82931-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KHSRP antibody (82931-5-RR, Clone: 230189A2 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82931-5-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 82931-5-RR (same clone as 82931-5-PBS)

    FC experiment of HeLa using 82931-5-RR (same clone as 82931-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.2 ug KHSRP Recombinant antibody (82931-5-RR, Clone:230189A2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82931-5-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 82931-5-RR (same clone as 82931-5-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 82931-5-RR (same clone as 82931-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82931-5-RR (KHSRP antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82931-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KHSRP antibody included
  • Affinity and Kinetic Characterization of 82931-5-RR

    Affinity and Kinetic Characterization of 82931-5-RR

    Biolayer interferometry (BLl) kinetic assays of 82931-5-RR against Human KHSRP were performed. The affinity constant is 2.11 nM.

KHSRP Recombinant monoclonal antibody, PBS Only

KHSRP Uni-rAb® Recombinant Antibody for IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82931-5-PBS

产品说明书

CloneNo. 230189A2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

KH type-splicing regulatory protein, FUSE-binding protein 2, FUBP2, FBP2, Far upstream element-binding protein 2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy KHSRP Ready-To-Use IHC Kit. KHSRP primary antibody included.

产品信息

82931-5-PBS targets KHSRP in IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag33890

Product name: Recombinant human KHSRP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 600-710 aa of NM_003685

Sequence: APPAQGEPPQPPPTGQSDYTKAWEEYYKKIGQQPQQPGAPPQQDYTKAWEEYYKKQAQVATGGGPGAPPGSQPDYSAAWAEYYRQQAAYYGQTPGPGGPQPPPTQQGQQQAQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 KH-type splicing regulatory protein
别名 KH type-splicing regulatory protein, FUSE-binding protein 2, FUBP2, FBP2, Far upstream element-binding protein 2
计算分子量73 kDa
GenBank蛋白编号NM_003685
基因名称 KHSRP
Gene ID (NCBI) 8570
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ92945
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

KHSRP, also named as FUBP2, KSRP, and p75, belongs to the KHSRP family. It binds to the dendritic targeting element and may play a role in mRNA trafficking. KHSRP is a part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. It mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. KHSRP may interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. It is involved in the degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.

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