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验证数据展示

  • IF Staining of HeLa using 82931-5-RR

    IF Staining of HeLa using 82931-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KHSRP antibody (82931-5-RR, Clone: 230189A2 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • FC experiment of HeLa using 82931-5-RR

    FC experiment of HeLa using 82931-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.2 ug KHSRP Recombinant antibody (82931-5-RR, Clone:230189A2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IHC staining of human intrahepatic cholangiocarcinoma using 82931-5-RR

    IHC staining of human intrahepatic cholangiocarcinoma using 82931-5-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82931-5-RR (KHSRP antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KHSRP antibody included
  • IHC staining of human rectal cancer using 82931-5-RR

    IHC staining of human rectal cancer using 82931-5-RR

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 82931-5-RR (KHSRP antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KHSRP antibody included
  • Affinity and Kinetic Characterization of 82931-5-RR

    Affinity and Kinetic Characterization of 82931-5-RR

    Biolayer interferometry (BLl) kinetic assays of 82931-5-RR against Human KHSRP were performed. The affinity constant is 2.11 nM.

KHSRP Recombinant monoclonal antibody

KHSRP Uni-rAb® Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 82931-5-RR

产品说明书

CloneNo. 230189A2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF/ICC, FC (Intra), ELISA

别名

230189A2, Far upstream element-binding protein 2, FBP2, FUBP2, FUSE-binding protein 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, mouse colon tissue, human rectal cancer tissue, mouse brain tissue, mouse kidney tissue, rat brain tissue, rat colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy KHSRP Ready-To-Use IHC Kit. KHSRP primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82931-5-RR targets KHSRP in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag33890

Product name: Recombinant human KHSRP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 600-710 aa of NM_003685

Sequence: APPAQGEPPQPPPTGQSDYTKAWEEYYKKIGQQPQQPGAPPQQDYTKAWEEYYKKQAQVATGGGPGAPPGSQPDYSAAWAEYYRQQAAYYGQTPGPGGPQPPPTQQGQQQAQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 KH-type splicing regulatory protein
别名 230189A2, Far upstream element-binding protein 2, FBP2, FUBP2, FUSE-binding protein 2
计算分子量73 kDa
GenBank蛋白编号NM_003685
基因名称 KHSRP
Gene ID (NCBI) 8570
RRIDAB_3670672
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ92945
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KHSRP, also named as FUBP2, KSRP, and p75, belongs to the KHSRP family. It binds to the dendritic targeting element and may play a role in mRNA trafficking. KHSRP is a part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. It mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. KHSRP may interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. It is involved in the degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.

实验方案

Product Specific Protocols
FC protocol for KHSRP antibody 82931-5-RRDownload protocol
IF protocol for KHSRP antibody 82931-5-RRDownload protocol
IHC protocol for KHSRP antibody 82931-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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