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验证数据展示

  • WB analysis using 31044-1-AP

    WB analysis using 31044-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 31044-1-AP (KHSRP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 31044-1-AP

    IF Staining of HeLa using 31044-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KHSRP antibody (31044-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IHC staining of human colon using 31044-1-AP

    IHC staining of human colon using 31044-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 31044-1-AP (KHSRP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KHSRP antibody included

KHSRP Polyclonal antibody

KHSRP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31044-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

Far upstream element-binding protein 2, FBP2, FUBP2, FUSE-binding protein 2, KH type-splicing regulatory protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inrat brain tissue
Positive IHC detected inhuman colon tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy KHSRP Ready-To-Use IHC Kit. KHSRP primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31044-1-AP targets KHSRP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 KHSRP fusion protein Ag33890 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 KH-type splicing regulatory protein
别名 Far upstream element-binding protein 2, FBP2, FUBP2, FUSE-binding protein 2, KH type-splicing regulatory protein
计算分子量73 kDa
观测分子量75-87kDa
GenBank蛋白编号NM_003685
基因名称 KHSRP
Gene ID (NCBI) 8570
RRIDAB_3669829
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92945
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KHSRP, also named as FUBP2, KSRP, and p75, belongs to the KHSRP family. It binds to the dendritic targeting element and may play a role in mRNA trafficking. KHSRP is a part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. It mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. KHSRP may interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. It is involved in the degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.

实验方案

Product Specific Protocols
WB protocol for KHSRP antibody 31044-1-APDownload protocol
IHC protocol for KHSRP antibody 31044-1-APDownload protocol
IF protocol for KHSRP antibody 31044-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols