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验证数据展示

  • IHC staining of human stomach cancer using 23443-1-AP

    IHC staining of human stomach cancer using 23443-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23443-1-AP (KHDC1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 23443-1-AP

    IHC staining of human stomach cancer using 23443-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23443-1-AP (KHDC1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of L02 using 23443-1-AP

    WB analysis of L02 using 23443-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 23443-1-AP (KHDC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 23443-1-AP

    IHC staining of human stomach using 23443-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 23443-1-AP (KHDC1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

KHDC1 Polyclonal antibody

KHDC1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 23443-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

NDG1, KHDC 1, KH homology domain-containing protein 1, C6orf148, C6orf147

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inL02 cells
Positive IHC detected inhuman stomach cancer tissue, human liver tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23443-1-AP targets KHDC1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag20115

Product name: Recombinant human KHDC1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 201-237 aa of BC022080

Sequence: DLSLAPRISGTVCLSVPQPSPYQVIGCSGFHLSSLYP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 KH homology domain containing 1
别名 NDG1, KHDC 1, KH homology domain-containing protein 1, C6orf148, C6orf147
计算分子量 237 aa, 27 kDa
观测分子量 35-40 kDa
GenBank蛋白编号BC022080
基因名称 KHDC1
Gene ID (NCBI) 80759
RRIDAB_2879280
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ4VXA5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KHDC1, also named as C6orf148, belongs to the KHDC1 family. KHDC1 has two isoforms with calculated MW 27 kDa and 18 kDa. It's a membrane protein. We got 35-40 kDa in our WB detection.

实验方案

Product Specific Protocols
WB protocol for KHDC1 antibody 23443-1-APDownload protocol
IHC protocol for KHDC1 antibody 23443-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols