验证数据展示
经过测试的应用
| Positive WB detected in | human placenta tissue, human testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32488-1-AP targets KCTD7 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | KCTD7 fusion protein Ag35756 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | potassium channel tetramerisation domain containing 7 |
| 别名 | BTB/POZ domain-containing protein KCTD7 |
| 计算分子量 | 33 kDa，289 aa |
| 观测分子量 | 62 kDa |
| GenBank蛋白编号 | NM_153033.4 |
| 基因名称 | KCTD7 |
| Gene ID (NCBI) | 154881 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q96MP8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KCTD7, or Potassium Channel Tetramerization Domain Containing 7, is a member of the KCTD family of proteins. The role of KCTD7 in regulating autophagy-lysosome pathways, which are essential for cellular homeostasis and response to nutrient stress (PMID: 36964131). It has been implicated in several neurodegenerative disorders, particularly progressive myoclonic epilepsy (PME) and neuronal ceroid lipofuscinosis (NCL). The deficiency of KCTD7 results in the accumulation of lipofuscin and lipid droplets, which are hallmarks of NCL (PMID: 33970744). The 62 kDa form most likely corresponds to an SDS-stable dimer (PMID: 22748208).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KCTD7 antibody 32488-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |