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验证数据展示

  • IHC staining of human colon cancer using 85210-4-RR

    IHC staining of human colon cancer using 85210-4-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85210-4-RR (KCTD5 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 85210-4-RR

    IHC staining of human ovary cancer using 85210-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 85210-4-RR (KCTD5 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 85210-4-RR

    IF Staining of HeLa using 85210-4-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using KCTD5 antibody (85210-4-RR, Clone: 242864A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

KCTD5 Recombinant monoclonal antibody

KCTD5 Uni-rAb® Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 85210-4-RR

产品说明书

CloneNo. 242864A11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, ELISA

别名

242864A11, BTB/POZ domain-containing protein KCTD5, KCTD17, KCTD2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman ovary cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85210-4-RR targets KCTD5 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag7915

Product name: Recombinant human KCTD5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-234 aa of BC007314

Sequence: MAENHCELLSPARGGIGAGLGGGLCRRCSAGLGALAQRPGSVSKWVRLNVGGTYFLTTRQTLCRDPKSFLYRLCQADPDLDSDKDETGAYLIDRDPTYFGPVLNYLRHGKLVINKDLAEEGVLEEAEFYNITSLIKLVKDKIRERDSKTSQVPVKHVYRVLQCQEEELTQMVSTMSDGWKFEQLVSIGSSYNYGNEDQAEFLCVVSKELHNTPYGTASEPSEKAKILQERGSRM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 potassium channel tetramerisation domain containing 5
别名 242864A11, BTB/POZ domain-containing protein KCTD5, KCTD17, KCTD2
计算分子量 26 kDa
GenBank蛋白编号BC007314
基因名称 KCTD5
Gene ID (NCBI) 54442
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9NXV2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KCTD5 belongs to the human potassium (K+) channel tetramerization domain (KCTD) family of proteins which share sequence similarity with the cytoplasmic domain of voltage-gated K+ channels (Kv channels). The KCTD proteins have relatively conserved N-terminal domains and variable C-termini (PMID: 24268103). KCTD5 interacts with cullin3 and has been proposed to function as substrate adapter for cullin3 based ubiquitin E3 ligases (PMID: 26188516).

实验方案

Product Specific Protocols
IF protocol for KCTD5 antibody 85210-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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