验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, C6 cells, HEK-293 cells, mouse lung tissue |
| Positive IP detected in | C6 cells |
| Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
产品信息
20128-1-AP targets KCTD15 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag13962 Product name: Recombinant human KCTD15 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-234 aa of BC001185 Sequence: MPHRKERPSGSSLHTHGSTGTAEGGNMSRLSLTRSPVSPLAAQGIPLPAQLTKSNAPVHIDVGSHMYTSSLATLTKYPDSRISRLFNGTEPIVLDSLKQHYFIDRDGEIFRYVLSFLRTSKLLLPDDFKDFSLLYEEARYYQLQPMVRELERWQQEQEQRRRSRACDCLVVRVTPDLGERIALSGEKALIEEVFPETGDVMCNSVNAGWNQDPTHVIRFPLNGYCRLNSVQDVL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | potassium channel tetramerisation domain containing 15 |
| 别名 | BTB/POZ domain-containing protein KCTD15, Potassium channel tetramerization domain-containing protein 15 |
| 计算分子量 | 283 aa, 32 kDa |
| 观测分子量 | 26 kDa |
| GenBank蛋白编号 | BC001185 |
| 基因名称 | KCTD15 |
| Gene ID (NCBI) | 79047 |
| RRID | AB_10646430 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96SI1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KCTD15 is a member of the KCTD family of proteins, which are involved in various biological processes. KCTD15 plays a role in embryonic development by regulating the neural crest domain. It inhibits neural crest induction by repressing the Wnt/β-catenin signaling pathway. KCTD15 is involved in the NF-κB signaling pathway, which is crucial for immune responses and inflammation. Its deregulation can influence the activation of the IKK-β enzyme complex, triggering the NF-κB pathway. KCTD15's expression levels in peripheral blood cells and leukemia cells suggest its potential as a biomarker for diagnosing and monitoring acute leukemias. Its distinct expression profile in different types of white blood cells highlights its importance in both physiological and pathological processes.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for KCTD15 antibody 20128-1-AP | Download protocol |
| IP protocol for KCTD15 antibody 20128-1-AP | Download protocol |
| WB protocol for KCTD15 antibody 20128-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncogenesis KCTD15 inhibits the Hedgehog pathway in Medulloblastoma cells by increasing protein levels of the oncosuppressor KCASH2.
| ||
Proc Natl Acad Sci U S A KCTD1 regulation of Adenylyl cyclase type 5 adjusts striatal cAMP signaling |
