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验证数据展示

  • WB analysis of mouse brain using 15523-1-AP (same clone as 15523-1-PBS)

    WB analysis of mouse brain using 15523-1-AP (same clone as 15523-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15523-1-AP (KCTD12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15523-1-PBS in a different storage buffer formulation.

  • IHC staining of human bowen disease using 15523-1-AP (same clone as 15523-1-PBS)

    IHC staining of human bowen disease using 15523-1-AP (same clone as 15523-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 15523-1-AP (KCTD12 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15523-1-PBS in a different storage buffer formulation.

  • IHC staining of human bowen disease using 15523-1-AP (same clone as 15523-1-PBS)

    IHC staining of human bowen disease using 15523-1-AP (same clone as 15523-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 15523-1-AP (KCTD12 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15523-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 15523-1-AP (same clone as 15523-1-PBS)

    IF Staining of HeLa using 15523-1-AP (same clone as 15523-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using KCTD12 antibody (15523-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 15523-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 15523-1-AP (same clone as 15523-1-PBS)

    IF Staining of MCF-7 using 15523-1-AP (same clone as 15523-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using KCTD12 antibody (15523-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 15523-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

KCTD12 Polyclonal antibody, PBS Only

KCTD12 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 15523-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

BTB/POZ domain-containing protein KCTD12, C13orf2, PFET1, Pfetin

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

15523-1-PBS targets KCTD12 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5716

Product name: Recombinant human KCTD12 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-325 aa of BC013764

Sequence: MALADSTRGLPNGGGGGGGSGSSSSSAEPPLFPDIVELNVGGQVYVTRRCTVVSVPDSLLWRMFTQQQPQELARDSKGRFFLDRDGFLFRYILDYLRDLQLVLPDYFPERSRLQREAEYFELPELVRRLGAPQQPGPGPPPSRRGVHKEGSLGDELLPLGYSEPEQQEGASAGAPSPTLELASRSPSGGAAGPLLTPSQSLDGSRRSGYITIGYRGSYTIGRDAQADAKFRRVARITVCGKTSLAKEVFGDTLNESRDPDRPPERYTSRYYLKFNFLEQAFDKLSESGFHMVACSSTGTCAFASSTDQSEDKIWTSYTEYVFCRE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 potassium channel tetramerisation domain containing 12
别名 BTB/POZ domain-containing protein KCTD12, C13orf2, PFET1, Pfetin
计算分子量 36 kDa
观测分子量 36 kDa
GenBank蛋白编号BC013764
基因名称 KCTD12
Gene ID (NCBI) 115207
RRIDAB_2132006
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96CX2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

KCTD12 also known as PFET1 or PFETIN, which contains a voltage-gated potassium (K+) channel tetramerization T1 domain and a BTB/POZ (Bric-a-brac, Tram-track, Broad complex poxvirus and zinc finger) domain, belongs to the KCTD family and was initially identified in cochlea. In addition to being a K+ channel protein that responds to membrane potential7, KCTD12 also acts as an auxiliary subunit of GABAB (γ-aminobutyric acid type B) receptors, which regulate emotionality and neuronal excitability.

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