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验证数据展示

  • WB analysis using 84203-5-RR

    WB analysis using 84203-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84203-5-RR (KCNJ18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skin using 84203-5-RR

    IHC staining of mouse skin using 84203-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 84203-5-RR (KCNJ18 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse skin using 84203-5-RR

    IF Staining of mouse skin using 84203-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using KCNJ18 antibody (84203-5-RR, Clone: 241381A2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84203-5-RR

    Affinity and Kinetic Characterization of 84203-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84203-5-RR against Human KCNJ18 were performed. The affinity constant is below 3.06 nM.

KCNJ18 Recombinant monoclonal antibody

KCNJ18 Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 84203-5-RR

产品说明书

CloneNo. 241381A2

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

TTPP2, KIR2.6, Inward rectifier potassium channel 18, 241381A2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse skin tissue, rat skin tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inmouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse skin tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84203-5-RR targets KCNJ18 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Ag34177

Product name: Recombinant human KCNJ18 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 353-433 aa of NM_001194958

Sequence: STPRCSAKDLVENKFLLPSANSFCYENELAFLSRDEEDEADGDQDGRSRDGLSPQARHDFDRLQAGGGVLEQRPYRRGSEI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 similar to hkir2.2x
别名 TTPP2, KIR2.6, Inward rectifier potassium channel 18, 241381A2
计算分子量 49 kDa
观测分子量45 kDa
GenBank蛋白编号NM_001194958
基因名称 KCNJ18
Gene ID (NCBI) 100134444
RRIDAB_3671759
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDB7U540
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mutations in KCNJ18 gene encoding an inwardly rectifying potassium channel (Kir2.6) cause thyrotoxic periodic paralysis (TPP) and sporadic, that is, non-familial cases of HypoPP. Mutant Kir2.6 proteins form a heterotetrameric Kir channel complex with Kir2.1 and thereby reduce cell surface expression of the complex. (PMID: 25882930)

实验方案

Product Specific Protocols
IF protocol for KCNJ18 antibody 84203-5-RRDownload protocol
IHC protocol for KCNJ18 antibody 84203-5-RRDownload protocol
WB protocol for KCNJ18 antibody 84203-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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