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验证数据展示

  • WB analysis of mouse skin using 30911-1-AP (same clone as 30911-1-PBS)

    WB analysis of mouse skin using 30911-1-AP (same clone as 30911-1-PBS)

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 30911-1-AP (KCNJ18 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30911-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse skin using 30911-1-AP (same clone as 30911-1-PBS)

    IF Staining of mouse skin using 30911-1-AP (same clone as 30911-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using KCNJ18 antibody (30911-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30911-1-PBS in a different storage buffer formulation.

KCNJ18 Polyclonal antibody, PBS Only

KCNJ18 Polyclonal Antibody for WB, IF-P, Indirect ELISA
Cat No. 30911-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF-P, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

30911-1-PBS targets KCNJ18 in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag34177

Product name: Recombinant human KCNJ18 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 353-433 aa of NM_001194958

Sequence: STPRCSAKDLVENKFLLPSANSFCYENELAFLSRDEEDEADGDQDGRSRDGLSPQARHDFDRLQAGGGVLEQRPYRRGSEI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 similar to hkir2.2x
别名
计算分子量 49 kDa
观测分子量45 kDa
GenBank蛋白编号NM_001194958
基因名称 KCNJ18
Gene ID (NCBI) 100134444
RRIDAB_3669782
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDB7U540
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mutations in KCNJ18 gene encoding an inwardly rectifying potassium channel (Kir2.6) cause thyrotoxic periodic paralysis (TPP) and sporadic, that is, non-familial cases of HypoPP. Mutant Kir2.6 proteins form a heterotetrameric Kir channel complex with Kir2.1 and thereby reduce cell surface expression of the complex. (PMID: 25882930)

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