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验证数据展示

  • WB analysis of mouse skin using 30911-1-AP

    WB analysis of mouse skin using 30911-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 30911-1-AP (KCNJ18 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse skin using 30911-1-AP

    IF Staining of mouse skin using 30911-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using KCNJ18 antibody (30911-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

KCNJ18 Polyclonal antibody

KCNJ18 Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 30911-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, IF-P, ELISA

别名

KCNJ18, LOC100134444, similar to hkir2.2x

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse skin tissue
Positive IF-P detected inmouse skin tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30911-1-AP targets KCNJ18 in WB, IF-P, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 Human, mouse
免疫原

CatNo: Ag34177

Product name: Recombinant human KCNJ18 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 353-433 aa of NM_001194958

Sequence: STPRCSAKDLVENKFLLPSANSFCYENELAFLSRDEEDEADGDQDGRSRDGLSPQARHDFDRLQAGGGVLEQRPYRRGSEI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 similar to hkir2.2x
别名 KCNJ18, LOC100134444, similar to hkir2.2x
计算分子量 49 kDa
观测分子量45 kDa
GenBank蛋白编号NM_001194958
基因名称 KCNJ18
Gene ID (NCBI) 100134444
RRIDAB_3669782
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDB7U540
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mutations in KCNJ18 gene encoding an inwardly rectifying potassium channel (Kir2.6) cause thyrotoxic periodic paralysis (TPP) and sporadic, that is, non-familial cases of HypoPP. Mutant Kir2.6 proteins form a heterotetrameric Kir channel complex with Kir2.1 and thereby reduce cell surface expression of the complex. (PMID: 25882930)

实验方案

Product Specific Protocols
IF protocol for KCNJ18 antibody 30911-1-APDownload protocol
WB protocol for KCNJ18 antibody 30911-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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