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KChIP1 Monoclonal antibody

KChIP1 Monoclonal Antibody for IF-P, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

3D6C1

Cat No : 66439-1-Ig

Print datasheet

Synonyms

KCHIP1, KCNIP1, VABP, Vesicle APC binding protein

验证数据展示

  • WB analysis of human cerebellum using 66439-1-Ig

    WB analysis of human cerebellum using 66439-1-Ig

    human cerebellum tissue were subjected to SDS PAGE followed by western blot with 66439-1-Ig (KChIP1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 66439-1-Ig

    IHC staining of human brain using 66439-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66439-1-Ig (KChIP1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 66439-1-Ig

    IHC staining of human brain using 66439-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66439-1-Ig (KChIP1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 66439-1-Ig

    IF Staining of mouse brain using 66439-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using KChIP1 antibody (66439-1-Ig, Clone: 3D6C1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of mouse brain using 66439-1-Ig

    IF Staining of mouse brain using 66439-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using KChIP1 antibody (66439-1-Ig, Clone: 3D6C1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inhuman cerebellum tissue, fetal human brain tissue, human spleen tissue
Positive IHC detected inhuman brain tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66439-1-Ig targets KChIP1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF-P, IHC, WB, ELISA
Tested Reactivity human, mouse
Immunogen KChIP1 fusion protein Ag5494 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name Kv channel interacting protein 1
Synonyms KCHIP1, KCNIP1, VABP, Vesicle APC binding protein
Calculated Molecular Weight 227 aa, 27 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC050375
Gene Symbol KCNIP1
Gene ID (NCBI) 30820
RRIDAB_2881809
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9NZI2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human K(v) channel interacting protein 1 (KCHIP1) is a new member of the neural calcium binding protein superfamily. Members of the KCNIP family are small calcium binding proteins. They all have EF-hand-like domains, and differ from each other in the N-terminus. They are integral subunit components of native Kv4 channel complexes. They may regulate A-type currents, and hence neuronal excitability, in response to changes in intracellular calcium. KChIP1 is a neuronal calcium sensor protein that is predominantly expressed at GABAergic synapses and it has been related with modulation of K(+) channels, GABAergic transmission and cell death.

实验方案

Product Specific Protocols
WB protocol for KChIP1 antibody 66439-1-IgDownload protocol
IHC protocol for KChIP1 antibody 66439-1-IgDownload protocol
IF protocol for KChIP1 antibody 66439-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols