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KCNAB1 Polyclonal antibody

KCNAB1 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 14697-1-AP

Print datasheet

Synonyms

AKR6A3, hKvb3, hKvBeta3, K(+) channel subunit beta 1, KCNA1B, KCNAB1, KV BETA 1, Kvb1.3

验证数据展示

  • WB analysis of mouse heart using 14697-1-AP

    WB analysis of mouse heart using 14697-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 14697-1-AP (KCNAB1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 14697-1-AP

    IHC staining of human brain using 14697-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 14697-1-AP (KCNAB1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 14697-1-AP

    IHC staining of human brain using 14697-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 14697-1-AP (KCNAB1 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HeLa using 14697-1-AP

    IF Staining of HeLa using 14697-1-AP

    Immunofluorescent analysis of Hela cells, using KCNAB1 antibody 14697-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

经过测试的应用

Positive WB detected inmouse heart tissue, Neuro-2a cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1500
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14697-1-AP targets KCNAB1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen KCNAB1 fusion protein Ag6339 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name potassium voltage-gated channel, shaker-related subfamily, beta member 1
Synonyms AKR6A3, hKvb3, hKvBeta3, K(+) channel subunit beta 1, KCNA1B, KCNAB1, KV BETA 1, Kvb1.3
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC043166
Gene Symbol KCNAB1
Gene ID (NCBI) 7881
RRIDAB_2131349
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14722
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KCNAB1 antibody 14697-1-APDownload protocol
IHC protocol for KCNAB1 antibody 14697-1-APDownload protocol
IF protocol for KCNAB1 antibody 14697-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols