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验证数据展示

  • WB analysis using 31900-1-AP

    WB analysis using 31900-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31900-1-AP (KCNA6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 31900-1-AP

    IHC staining of mouse brain using 31900-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31900-1-AP (KCNA6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 31900-1-AP

    IHC staining of rat brain using 31900-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 31900-1-AP (KCNA6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

KCNA6 Polyclonal antibody

KCNA6 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31900-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

HBK2, Human Brain Potassium Channel-2, Kv1.6, Potassium voltage-gated channel subfamily A member 6, PPP1R96

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inDaudi cells, Jurkat cells, Raji cells, TT cells, U2OS cells
Positive IHC detected inmouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31900-1-AP targets KCNA6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 KCNA6 fusion protein Ag35654 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 potassium voltage-gated channel, shaker-related subfamily, member 6
别名 HBK2, Human Brain Potassium Channel-2, Kv1.6, Potassium voltage-gated channel subfamily A member 6, PPP1R96
计算分子量59 kDa，529aa
观测分子量65 kDa
GenBank蛋白编号NM_002235.3
基因名称 KCNA6
Gene ID (NCBI) 3742
RRIDAB_3670137
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP17658
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Shaker-like Kv1 potassium channel family comprises 8 α subunits (Kv1.1-Kv1.8), which are key regulators of neuronal excitability in mammals (PMID: 7842011; 9581771; 22612818). Potassium voltage-gated channel subfamily A member 6 (KCNA6, also known as HBK2, Kv1.6, and PPP1R96) is a multi-pass membrane protein that is mainly expressed in the central and peripheral nervous system (PMID: 12042352; 8872310). The concentration of the Kv1.6 channel in the cell membrane can modulate the kinetic properties of Kv1.6-induced K+ current (PMID: 14575698).

实验方案

Product Specific Protocols
WB protocol for KCNA6 antibody 31900-1-APDownload protocol
IHC protocol for KCNA6 antibody 31900-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols