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验证数据展示

  • WB analysis using 31900-1-AP

    WB analysis using 31900-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31900-1-AP (KCNA6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 31900-1-AP

    IHC staining of mouse brain using 31900-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31900-1-AP (KCNA6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 31900-1-AP

    IHC staining of rat brain using 31900-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 31900-1-AP (KCNA6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse cerebellum using 31900-1-AP

    IF Staining of mouse cerebellum using 31900-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using KCNA6 antibody (31900-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), FUS/TLS antibody (68262-1-Ig, Clone: 1B4F8, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

KCNA6 Polyclonal antibody

KCNA6 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 31900-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

HBK2, Human Brain Potassium Channel-2, Kv1.6, Potassium voltage-gated channel subfamily A member 6, PPP1R96

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inDaudi cells, Jurkat cells, Raji cells, TT cells, U2OS cells
Positive IHC detected inmouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31900-1-AP targets KCNA6 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag35654

Product name: Recombinant human KCNA6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 194-262 aa of NM_002235.3

Sequence: ETLPQFRVDGRGGNNGGVSRVSPVSRGSQEEEEDEDDSYTFHHGITPGEMGTGGSSSLSTLGGSFFTDP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 potassium voltage-gated channel, shaker-related subfamily, member 6
别名 HBK2, Human Brain Potassium Channel-2, Kv1.6, Potassium voltage-gated channel subfamily A member 6, PPP1R96
计算分子量59 kDa，529aa
观测分子量65 kDa
GenBank蛋白编号NM_002235.3
基因名称 KCNA6
Gene ID (NCBI) 3742
RRIDAB_3670137
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP17658
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Shaker-like Kv1 potassium channel family comprises 8 α subunits (Kv1.1-Kv1.8), which are key regulators of neuronal excitability in mammals (PMID: 7842011; 9581771; 22612818). Potassium voltage-gated channel subfamily A member 6 (KCNA6, also known as HBK2, Kv1.6, and PPP1R96) is a multi-pass membrane protein that is mainly expressed in the central and peripheral nervous system (PMID: 12042352; 8872310). The concentration of the Kv1.6 channel in the cell membrane can modulate the kinetic properties of Kv1.6-induced K+ current (PMID: 14575698).

实验方案

Product Specific Protocols
IHC protocol for KCNA6 antibody 31900-1-APDownload protocol
WB protocol for KCNA6 antibody 31900-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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