• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

KBTBD2 Monoclonal antibody

KBTBD2 Monoclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

IF/ICC, WB, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

2A9F3

Cat No : 67895-1-Ig

Print datasheet

Synonyms

BKLHD1, KBTBD2, KIAA1489

验证数据展示

  • WB analysis using 67895-1-Ig

    WB analysis using 67895-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67895-1-Ig (KBTBD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of HeLa using 67895-1-Ig

    IF Staining of HeLa using 67895-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using KBTBD2 antibody (67895-1-Ig, Clone: 2A9F3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). DAPI (blue).

经过测试的应用

Positive WB detected inA549 cells, U2OS cells, HeLa cells, K-562 cells, Jurkat cells, HSC-T6 cells
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67895-1-Ig targets KBTBD2 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen KBTBD2 fusion protein Ag4933 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name kelch repeat and BTB (POZ) domain containing 2
Synonyms BKLHD1, KBTBD2, KIAA1489
Calculated Molecular Weight 622 aa, 71 kDa
Observed Molecular Weight60-70 kDa
GenBank Accession NumberBC047107
Gene Symbol KBTBD2
Gene ID (NCBI) 25948
RRIDAB_2918651
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8IY47
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Kelch repeat and BTB domain containing 2(KBTBD2), also known as BTB and kelch domain-containing protein 1, encodes a BTB-Kelch family subunit that p85α-specific recognition for the Cullin3-based E3 ubiqitin ligase complex, which regulates p85α protein ubiquitination and subsequently regulates phosphoinositide-3 kinases (PI3K) signaling (PMID:27708159). However, the expression profile and functional significance of Kbtbd2 is still largely unknown. The Human Protein Atlas which is an online source shows high Kbtbd2 expression in the spleen and thymus, suggesting that Kbtbd2 might involved in immunization (PMID:29331106). In addition, Kbtbd2 is related to Src-induced phosphorylation of protein, spermatogonial stem cell activity(PMID:15623525, 29331106).

实验方案

Product Specific Protocols
WB protocol for KBTBD2 antibody 67895-1-IgDownload protocol
IF protocol for KBTBD2 antibody 67895-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC

Pathol Res Pract

KBTBD2 promotes proliferation and migration of gastric cancer via activating EGFR signaling pathway

Authors - Jishuang Ding
  • KD Validated
View Article