验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, Jurkat cells, HeLa cells |
| Positive IP detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20166-1-AP targets KATNAL1 in WB, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag13960 Product name: Recombinant human KATNAL1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-261 aa of BC000612 Sequence: MNLAEICDNAKKGREYALLGNYDSSMVYYQGVMQQIQRHCQSVRDPAIKGKWQQVRQELLEEYEQVKSIVSTLESFKIDKPPDFPVSCQDEPFRDPAVWPPPVPAEHRAPPQIRRPNREVRPLRKEMAGVGARGPVGRAHPISKSEKPSTSRDKDYRARGRDDKGRKNMQDGASDGEMPKFDGAGYDKDLVEALERDIVSRNPSIHWDDIADLEEAKKLLREAVVLPMWMPDFFKGIRRPWKGVLMVGPPGTGKTMLAKAV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | katanin p60 subunit A-like 1 |
| 别名 | p60 katanin-like 1, p60 katanin like 1, Katanin p60 subunit A-like 1, katanin p60 subunit A like 1, Katanin p60 ATPase-containing subunit A-like 1 |
| 计算分子量 | 490 aa, 55 kDa |
| 观测分子量 | 50-55 kDa |
| GenBank蛋白编号 | BC000612 |
| 基因名称 | KATNAL1 |
| Gene ID (NCBI) | 84056 |
| RRID | AB_2878651 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BW62 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KATNAL1 belongs to a family of microtubule-severing proteins and contains an N-terminal microtubule interaction and trafficking (MIT) domain, an ATPase AAA-type core domain, and a C-terminal Vps4_C domain. KATNAL1 is involved in regulating microtubule dynamics, which is essential for spermiogenesis and male fertility. It severs microtubules in an ATP-dependent manner, promoting rapid reorganization of cellular microtubule arrays(PMID: 22654668). In neurons, KATNAL1 plays a role in microtubule rearrangement during neuronal migration and process elongation. Additionally, mutations in KATNAL1 have been associated with intellectual disability and microcephaly in humans(PMID: 30448058).
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for KATNAL1 antibody 20166-1-AP | Download protocol |
| WB protocol for KATNAL1 antibody 20166-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
