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验证数据展示

  • FC experiment of U2OS using CL488-66575

    FC experiment of U2OS using CL488-66575

    1x10^6 U2OS cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Kat2a/Gcn5 Monoclonal Antibody (CL488-66575, Clone:1F6G7)(red), or 0.4 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated KAT2A/GCN5 Monoclonal antibody

KAT2A/GCN5 Monoclonal Antibody for FC (Intra)
Cat No. CL488-66575

产品说明书

CloneNo. 1F6G7

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

FC (Intra)

别名

General control of amino acid synthesis protein 5-like 2, GCN5L2, GCN5, EC:2.3.1.48, EC:2.3.1.-

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inU2OS cells

This antibody may not be suitable for IHC test.

For other applications, we recommend the unconjugated version of this antibody, 66575-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66575 targets KAT2A/GCN5 in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 KAT2A/GCN5 fusion protein Ag6940 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 K(lysine) acetyltransferase 2A
别名 General control of amino acid synthesis protein 5-like 2, GCN5L2, GCN5, EC:2.3.1.48, EC:2.3.1.-
计算分子量 94 kDa
观测分子量 94 kDa
GenBank蛋白编号BC032743
基因名称 KAT2A
Gene ID (NCBI) 2648
RRIDAB_3672916
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ92830
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GCN5, encoded by KAT2A, is a member of nuclear A-type histone acetyltransferase (HAT) which has direct impact on gene transcription. Additionally, GCN5 has roles in cell cycle regulation, DNA replication, and DNA repair, highlighting it as key player in the maintenance of genome stability. GCN5 is a coactivator of c-MYC oncogene protein and has oncogenic roles in various cancers.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 KAT2A/GCN5 antibody CL488-66575Download protocol
Standard Protocols
Click here to view our Standard Protocols