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验证数据展示

  • WB analysis of HeLa using 66630-1-Ig (same clone as 66630-1-PBS)

    WB analysis of HeLa using 66630-1-Ig (same clone as 66630-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66630-1-Ig (KAP1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66630-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 66630-1-Ig (same clone as 66630-1-PBS)

    WB analysis of HepG2 using 66630-1-Ig (same clone as 66630-1-PBS)

    WB result of KAP1 antibody (66630-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KAP1 transfected HepG2 cells. This data was developed using the same antibody clone with 66630-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 66630-1-Ig (same clone as 66630-1-PBS)

    IP experiment of HeLa using 66630-1-Ig (same clone as 66630-1-PBS)

    IP result of anti-KAP1 (IP:66630-1-Ig, 5ug; Detection:66630-1-Ig 1:1000) with HeLa cells lysate 4000 ug. This data was developed using the same antibody clone with 66630-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66630-1-Ig (same clone as 66630-1-PBS)

    IHC staining of human breast cancer using 66630-1-Ig (same clone as 66630-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66630-1-Ig (KAP1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66630-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KAP1 antibody included
  • IHC staining of human colon cancer using 66630-1-Ig (same clone as 66630-1-PBS)

    IHC staining of human colon cancer using 66630-1-Ig (same clone as 66630-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66630-1-Ig (KAP1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66630-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KAP1 antibody included
  • IF Staining of HepG2 using 66630-1-Ig (same clone as 66630-1-PBS)

    IF Staining of HepG2 using 66630-1-Ig (same clone as 66630-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KAP1 antibody (66630-1-Ig, Clone: 1B9G12 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 66630-1-PBS in a different storage buffer formulation.

  • WB analysis using 66630-1-Ig (same clone as 66630-1-PBS)

    WB analysis using 66630-1-Ig (same clone as 66630-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66630-1-Ig (KAP1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66630-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

KAP1 Monoclonal antibody, PBS Only

KAP1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 66630-1-PBS

产品说明书

CloneNo. 1B9G12

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

TRIM28, KRAB associated protein 1, KAP-1, KAP 1, EC:2.3.2.27

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy KAP1 Ready-To-Use IHC Kit. KAP1 primary antibody included.

产品信息

66630-1-PBS targets KAP1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原 KAP1 fusion protein Ag7519 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 tripartite motif-containing 28
别名 TRIM28, KRAB associated protein 1, KAP-1, KAP 1, EC:2.3.2.27
计算分子量 89 kDa
观测分子量 100 kDa
GenBank蛋白编号BC004978
基因名称 KAP1
Gene ID (NCBI) 10155
RRIDAB_2732886
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13263
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

KAP1, also named as TRIM28 or RNF96, is a 835 amino acid protein, which contain one RING-type zinc finger, one PHD-type zinc finger, one bromo domain and two B box-type zinc fingers. KAP1 localizes in the nucleus and Belongs to the TRIM/RBCC family. KAP1 is a nuclear corepressor for KRAB domain-containing zinc finger proteins and mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 to the promoter regions of KRAB target genes. KAP1 is expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. The calcualted molecular weight of KAP1 is 89 kDa, but modified KAP1 is about 100 kDa. (PMID: 18590578 )