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- KD/KO Validated
KAP1 Monoclonal antibody
KAP1 Monoclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human and More (2)
Applications
IF/ICC, IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
1B9G12
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HepG2 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human breast cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:20000-1:100000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66630-1-Ig targets KAP1 in WB, IP, IF, IHC, ChIP, ELISA applications and shows reactivity with Human samples.
Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
Cited Applications | WB, IF, ChIP |
Tested Reactivity | Human |
Cited Reactivity | human, mouse |
Immunogen | KAP1 fusion protein Ag7519 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | tripartite motif-containing 28 |
Synonyms | KAP 1, KAP1, KRAB associated protein 1, KRAB interacting protein 1, KRIP 1, Nuclear corepressor KAP 1, RING finger protein 96, RNF96, TF1B, TIF1 beta, TIF1B, TRIM28, tripartite motif containing 28 |
Calculated Molecular Weight | 89 kDa |
Observed Molecular Weight | 100 kDa |
GenBank Accession Number | BC004978 |
Gene Symbol | TRIM28 |
Gene ID (NCBI) | 10155 |
RRID | AB_2732886 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q13263 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for KAP1 antibody 66630-1-Ig | Download protocol |
IHC protocol for KAP1 antibody 66630-1-Ig | Download protocol |
IF protocol for KAP1 antibody 66630-1-Ig | Download protocol |
IP protocol for KAP1 antibody 66630-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Sci Adv Epac1 activation by cAMP regulates cellular SUMOylation and promotes the formation of biomolecular condensates. | ||
Nucleic Acids Res SIRT6 coordinates with CHD4 to promote chromatin relaxation and DNA repair. | ||
BMC Pulm Med miR-125b-5p upregulation by TRIM28 induces cisplatin resistance in non-small cell lung cancer through CREB1 inhibition
| ||
Open Biol A critical threshold of MCM10 is required to maintain genome stability during differentiation of induced pluripotent stem cells into natural killer cells | ||