验证数据展示
经过测试的应用
| Positive IHC detected in | human colon tissue, human kidney tissue, mouse heart tissue, mouse ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy KANK2 Ready-To-Use IHC Kit. KANK2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
20546-1-AP targets KANK2 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | KN motif and ankyrin repeat domains 2 |
| 别名 | MXRA3, Matrix-remodeling-associated protein 3, KN motif and ankyrin repeat domain-containing protein 2, KIAA1518, Ankyrin repeat domain-containing protein 25 |
| 计算分子量 | 91 kDa |
| GenBank蛋白编号 | NM_001136191 |
| 基因名称 | KANK2 |
| Gene ID (NCBI) | 25959 |
| RRID | AB_10694286 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q63ZY3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KANK2, also named as ANKRD25, KIAA1518, MXRA3 and SIP, is found as a candidate tumor suppressor for renal cell carcinoma. It is an ankyrin-repeat domain-containing protein. KANK2 has an identical role in the formation of actin stress fibers. The antibody is specific to KANK2.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for KANK2 antibody 20546-1-AP | Download protocol |
| IHC protocol for KANK2 antibody 20546-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
