验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HT-29 cells |
Mouse monoclonal antibodies of IgA isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66890-1-Ig targets Jagged1/CD339 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat, goat |
| 免疫原 |
CatNo: Ag11891 Product name: Recombinant human Jagged1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 35-350 aa of BC098393 Sequence: FELEILSMQNVNGELQNGNCCGGARNPGDRKCTRDECDTYFKVCLKEYQSRVTAGGPCSFGSGSTPVIGGNTFNLKASRGNDRNRIVLPFSFAWPRSYTLLVEAWDSSNDTVQPDSIIEKASHSGMINPSRQWQTLKQNTGVAHFEYQIRVTCDDYYYGFGCNKFCRPRDDFFGHYACDQNGNKTCMEGWMGPECNRAICRQGCSPKHGSCKLPGDCRCQYGWQGLYCDKCIPHPGCVHGICNEPWQCLCETNWGGQLCDKDLNYCGTHQPCLNGGTCSNTGPDKYQCSCPEGYSGPNCEIAEHACLSDPCHNRGS 种属同源性预测 |
| 宿主/亚型 | Mouse / IgA |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | jagged 1 (Alagille syndrome) |
| 别名 | JAG1, Jagged1, 2A7D11, CD339, HJ1 |
| 计算分子量 | 1218 aa, 134 kDa |
| 观测分子量 | 150 kDa |
| GenBank蛋白编号 | BC098393 |
| 基因名称 | Jagged1 |
| Gene ID (NCBI) | 182 |
| RRID | AB_2882220 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Thiophilic affinity chromatograph |
| UNIPROT ID | P78504 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Jagged1 is a type I, membrane- anchored, multidomain protein and a ligand of the Notch receptor. The receptor-ligand interaction can trigger the Notch signaling and activate transcription factors that play key roles in cell differentiation and morphogenesis. The predicted MW of Jagged1 is 134 kDa, while higher MW around 150-180 kDa had been observed in literations (PMID: 9716576, 9596653).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Jagged1/CD339 antibody 66890-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) The Loss of an Orphan Nuclear Receptor NR2E3 Augments Wnt/β-catenin Signaling via Epigenetic Dysregulation that Enhances Sp1-β catenin-p300 Interactions in Hepatocellular Carcinoma | ||
Cell Rep Med LIFUS-driven engineered bacteria reprogram immunosuppressive niches via mechano-NOTCH signaling. | ||
Int J Biol Sci Linc-KILH potentiates Notch1 signaling through inhibiting KRT19 phosphorylation and promotes the malignancy of hepatocellular carcinoma. | ||
Cell Death Discov Molecular mechanism underlying miR-204-5p regulation of adipose-derived stem cells differentiation into cells from three germ layers | ||
Cell Syst Dynamic decoding of VEGF signaling and coordinated control of multiple phenotypes by the Src-TEM4-YAP pathway |
