验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells |
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | A431 cells |
Positive FC detected in | A431 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:6000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
83134-6-RR targets JUND in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with Human samples.
经测试应用 | WB, IF/ICC, FC (Intra), ELISA Application Description |
经测试反应性 | Human |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | jun D proto-oncogene |
别名 | AP 1, jun D proto oncogene, JUND, Transcription factor jun D |
计算分子量 | 35 kDa |
观测分子量 | 35-45 kDa |
GenBank蛋白编号 | NM_005354 |
基因名称 | JUND |
Gene ID (NCBI) | 3727 |
RRID | AB_3670836 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purfication |
UNIPROT ID | P17535 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for JUND antibody 83134-6-RR | Download protocol |
IF protocol for JUND antibody 83134-6-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |