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验证数据展示

  • ChIP experiment of HCT 116 using 83134-6-RR

    ChIP experiment of HCT 116 using 83134-6-RR

    Chromatin was prepared from HCT 116 cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of JUND (83134-6-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

  • WB analysis using 83134-6-RR

    WB analysis using 83134-6-RR

    HeLa cells were subjected to SDS PAGE followed by western blot with 83134-6-RR (JUND antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 83134-6-RR

    IF Staining of HeLa using 83134-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using JUND antibody (83134-6-RR, Clone: 230457A4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • FC experiment of A431 using 83134-6-RR

    FC experiment of A431 using 83134-6-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug JUND Recombinant antibody (83134-6-RR, Clone:230457A4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

JUND Recombinant monoclonal antibody

JUND Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA, ChIP-qPCR
Cat No. 83134-6-RR

产品说明书

CloneNo. 230457A4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA, ChIP-qPCR

别名

230457A4, AP 1, jun D proto oncogene, Transcription factor jun D

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inA431 cells
Positive ChIP-qPCR detected inHCT 116 cells
Planning an IHC experiment? We recommend our IHCeasy JUND Ready-To-Use IHC Kit. JUND primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
CHIP-QPCRCHIP-QPCR : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83134-6-RR targets JUND in WB, IF/ICC, FC (Intra), ELISA, ChIP-qPCR applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA, ChIP-qPCR Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 jun D proto-oncogene
别名 230457A4, AP 1, jun D proto oncogene, Transcription factor jun D
计算分子量 35 kDa
观测分子量 35-45 kDa
GenBank蛋白编号NM_005354
基因名称 JUND
Gene ID (NCBI) 3727
RRIDAB_3670836
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP17535
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for JUND antibody 83134-6-RRDownload protocol
WB protocol for JUND antibody 83134-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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