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验证数据展示

  • IF Staining of HeLa using CL488-10486

    IF Staining of HeLa using CL488-10486

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 JUNB antibody (CL488-10486) at dilution of 1:100.

  • FC experiment of MCF-7 using CL488-10486

    FC experiment of MCF-7 using CL488-10486

    1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 JUNB Polyclonal Antibody (CL488-10486)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated JUNB Polyclonal antibody

JUNB Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-10486

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

AP-1, AP 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 10486-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10486 targets JUNB in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 JUNB fusion protein Ag0752 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 jun B proto-oncogene
别名 AP-1, AP 1
计算分子量 36 kDa
观测分子量 42 kDa, 43 kDa
GenBank蛋白编号BC004250
基因名称 JUNB
Gene ID (NCBI) 3726
RRIDAB_3672444
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP17275
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

JunB is one of the components of the Activator Protein-1 (AP-1) transcription complex that have been implicated in the control ofthe G0/G1 transtion in fibroblasts. AP-1 is a collection of dimers formed by members of the Jun-, Fos-, ATF- and Maf multigene families that bind to specific DNA regulatory elements called AP-1/12-O-tetradecanoylphorbol-13-acetate-responsive elements (TREs) and cAMP-responsive elements (CREs). JunB binds to the DNA sequence 5'-TGA[CG]TCA-3', and involves in the regulation of gene activity following the primary growth factor response. It also acts either as a tumor suppressor or as an oncogene depending on the cell and physiopathological context

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 JUNB antibody CL488-10486Download protocol
Standard Protocols
Click here to view our Standard Protocols