• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84922-6-RR (same clone as 84922-6-PBS)

    WB analysis using 84922-6-RR (same clone as 84922-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84922-6-RR (JUNB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84922-6-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84922-6-RR (same clone as 84922-6-PBS)

    IF Staining of HeLa using 84922-6-RR (same clone as 84922-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using JUNB antibody (84922-6-RR, Clone: 242562G2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84922-6-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 84922-6-RR (same clone as 84922-6-PBS)

    FC experiment of HeLa using 84922-6-RR (same clone as 84922-6-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug JUNB Recombinant antibody (84922-6-RR, Clone:242562G2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84922-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84922-6-RR

    Affinity and Kinetic Characterization of 84922-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84922-6-RR against Human JUNB were performed. The affinity constant is 0.213 nM.

JUNB Recombinant antibody, PBS Only

JUNB Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84922-6-PBS

产品说明书

CloneNo. 242562G2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

AP-1, AP 1, 242562G2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

84922-6-PBS targets JUNB in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 JUNB fusion protein Ag0752 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 jun B proto-oncogene
别名 AP-1, AP 1, 242562G2
计算分子量 36 kDa
观测分子量 42 kDa, 43 kDa
GenBank蛋白编号BC004250
基因名称 JUNB
Gene ID (NCBI) 3726
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP17275
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

JunB is one of the components of the Activator Protein-1 (AP-1) transcription complex that have been implicated in the control ofthe G0/G1 transtion in fibroblasts. AP-1 is a collection of dimers formed by members of the Jun-, Fos-, ATF- and Maf multigene families that bind to specific DNA regulatory elements called AP-1/12-O-tetradecanoylphorbol-13-acetate-responsive elements (TREs) and cAMP-responsive elements (CREs). JunB binds to the DNA sequence 5'-TGA[CG]TCA-3', and involves in the regulation of gene activity following the primary growth factor response. It also acts either as a tumor suppressor or as an oncogene depending on the cell and physiopathological context