JIP1/IB-1 Polyclonal antibody

JIP1/IB-1 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 14568-1-AP

Print datasheet

Synonyms

IB 1, IB1, Islet brain 1, JIP 1, JIP1, JIP1/IB-1, JNK interacting protein 1, MAPK8IP1, PRKM8IP



经过测试的应用

Positive WB detected infetal human brain tissue
Positive IHC detected inhuman oesophagus cancer tissue, mouse brain tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-12 cells
Planning an IHC experiment? We recommend our IHCeasy MAPK8IP1/JIP1 Ready-To-Use IHC Kit. MAPK8IP1/JIP1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14568-1-AP targets JIP1/IB-1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB, IP, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen JIP1/IB-1 fusion protein Ag6150 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitogen-activated protein kinase 8 interacting protein 1
Synonyms IB 1, IB1, Islet brain 1, JIP 1, JIP1, JIP1/IB-1, JNK interacting protein 1, MAPK8IP1, PRKM8IP
Calculated Molecular Weight 78 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC068470
Gene Symbol MAPK8IP1
Gene ID (NCBI) 9479
RRIDAB_2281682
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UQF2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for JIP1/IB-1 antibody 14568-1-APDownload protocol
IHC protocol for JIP1/IB-1 antibody 14568-1-APDownload protocol
IF protocol for JIP1/IB-1 antibody 14568-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

ACS Chem Neurosci

Antidepressant-like Effect of Merazin Hydrate Depends on NO/ERK by Suppressing Its Downstream NF-κB or Nonactivating CREB/BDNF in Mouse Hippocampus.

Authors - Lei Wu
humanIP, WB

Front Physiol

Ras-Related C3 Botulinum Toxin Substrate 1 Combining With the Mixed Lineage Kinase 3- Mitogen-Activated Protein Kinase 7- c-Jun N-Terminal Kinase Signaling Module Accelerates Diabetic Nephropathy.

Authors - Changjiang Ying
mouseWB

Saudi Pharm J

iTRAQ-derived quantitative proteomics uncovers the neuroprotective property of bexarotene in a mice model of cerebral ischemia-reperfusion injury.

Authors - Hailin Liu
mouseWB

Cell Rep

SFRS11 Loss Leads to Aging-Associated Cognitive Decline by Modulating LRP8 and ApoE.

Authors - Obayed Raihan
humanWB

Oncotarget

Autophagy-related gene expression is an independent prognostic indicator of glioma.

Authors - Huixue Zhang
mouseIF

Sci Total Environ

Manganese disrupts the maturation and degradation of axonal autophagosome leading to hippocampal synaptic toxicity in mice via the activation of LRRK2 on phosphorylation of Rab10

Authors - Zhuo Ma