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验证数据展示

  • WB analysis using 34226-1-AP

    WB analysis using 34226-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 34226-1-AP (JAM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 34226-1-AP

    IHC staining of human placenta using 34226-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 34226-1-AP (JAM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human placenta using 34226-1-AP

    IF Staining of human placenta using 34226-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using JAM2 antibody (34226-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

JAM2 Polyclonal antibody

JAM2 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 34226-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

CD322, JAM-2, JAM-B, Junctional adhesion molecule 2, Junctional adhesion molecule B

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman placenta tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

34226-1-AP targets JAM2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Eg6328

Product name: Recombinant mouse CD322/JAM-B/VE-JAM protein

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 29-236 aa of NM_023844.5

Sequence: FSASKDHRQEVTVIEFQEAILACKTPKKTTSSRLEWKKVGQGVSLVYYQQALQGDFKDRAEMIDFNIRIKNVTRSDAGEYRCEVSAPTEQGQNLQEDKVMLEVLVAPAVPACEVPTSVMTGSVVELRCQDKEGNPAPEYIWFKDGTSLLGNPKGGTHNNSSYTMNTKSGILQFNMISKMDSGEYYCEARNSVGHRRCPGKRMQVDVLN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 junction adhesion molecule 2
别名 CD322, JAM-2, JAM-B, Junctional adhesion molecule 2, Junctional adhesion molecule B
计算分子量33 kDa
观测分子量38 kDa
GenBank蛋白编号NM_023844.5
基因名称 Jam2
Gene ID (NCBI) 67374
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9JI59
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Junctional adhesion molecules (JAMs) are integral membrane proteins belonging to the immunoglobulin (Ig) superfamily. JAMs are expressed by leukocytes, platelets, endothelial, and epithelial cells and localized at the tight junction of polarized cells and on the cell surface of leukocytes. JAM-2, also known as VE-JAM or JAM-B, is specifically expressed in lymphatic endothelial cells and endothelial venules. JAM2 regulates cell-cell adhesion and signaling by interacting with other tight junction proteins such as PAR-3 and ZO-1 (PMID: 14657270; 24595739; 11739175; 12953056).

实验方案

Product Specific Protocols
IF protocol for JAM2 antibody 34226-1-APDownload protocol
IHC protocol for JAM2 antibody 34226-1-APDownload protocol
WB protocol for JAM2 antibody 34226-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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