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验证数据展示

  • FC experiment of human peripheral blood leukocytes using Biotin-FcA98328-3

    FC experiment of human peripheral blood leukocytes using Biotin-FcA98328-3

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Biotin Anti-Human JAM-A/CD321 Rabbit RecAb (Biotin-FcA98328-3, Clone: 242290B3) or FcZero-rAb™ Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9), and Streptavidin-PE Conjugate (Cat.No. PE-PF00030). Cells were not fixed.

  • FC experiment of human peripheral blood leukocytes using Biotin-FcA98328-3

    FC experiment of human peripheral blood leukocytes using Biotin-FcA98328-3

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Biotin Anti-Human JAM-A/CD321 Rabbit RecAb (Biotin-FcA98328-3, Clone: 242290B3) (red) or FcZero-rAb™ Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9) (blue), and Streptavidin-PE Conjugate (Cat.No. PE-PF00030). Cells were not fixed.

  • Zero Fc receptor binding
  • Site-specific conjugation
  • One universal isotype

FcZero-rAb® Biotin Anti-Human JAM-A/CD321 Rabbit Recombinant Antibody

JAM-A/CD321 FcZero-rAb® Recombinant Antibody for FC, ELISA
Cat No. Biotin-FcA98328-3

产品说明书

CloneNo. 242290B3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC, ELISA

别名

F11R, JAM-A/Junctional Adhesion Molecule A, CD321, F11 receptor, JAM

缓冲液配方:  PBS, Azide
PBS, Azide
偶联物:  Biotin
规格价格库存

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经过测试的应用

Positive FC detected inhuman peripheral blood leukocytes

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-FcA98328-3 targets JAM-A/CD321 in FC, ELISA applications and shows reactivity with human samples.

经测试应用 FC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg1372

Product name: Recombinant Human JAM-A/Junctional Adhesion Molecule A protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 28-238 aa of NM_016946.6

Sequence: SVTVHSSEPEVRIPENNPVKLSCAYSGFSSPRVEWKFDQGDTTRLVCYNNKITASYEDRVTFLPTGITFKSVTREDTGTYTCMVSEEGGNSYGEVKVKLIVLVPPSKPTVNIPSSATIGNRAVLTCSEQDGSPPSEYTWFKDGIVMPTNPKSTRAFSNSSYVLNPTTGELVFDPLSASDTGEYSCEARNGYGTPMTSNAVRMEAVERNVGV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 F11 receptor
别名 F11R, JAM-A/Junctional Adhesion Molecule A, CD321, F11 receptor, JAM
计算分子量33kDa
GenBank蛋白编号NM_016946.6
基因名称 F11R
Gene ID (NCBI) 50848
偶联类型 Biotin
最大激发/发射波长-
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y624-1
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Junctional adhesion molecule A (JAM-A), also known as CD321 or F11 receptor (F11R), was first identified in human platelets as a 32/35 kDa protein duplex that serves as the receptor for a functional antibody that activates platelets. It seems to play a role in epithelial tight junction formation. It was also reported to function as a receptor for reovirus and a ligand for the integrin LFA1.

实验方案

Product Specific Protocols
FC protocol for Biotin JAM-A/CD321 antibody Biotin-FcA98328-3Download protocol
Standard Protocols
Click here to view our Standard Protocols
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