验证数据展示
经过测试的应用
| Positive WB detected in | Daudi cells, J774A.1 cells, RAW 264.7 cells, mouse lung tissue, rat lung tissue |
Planning an IHC experiment? We recommend our IHCeasy JAK1 Ready-To-Use IHC Kit. JAK1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27284-1-AP targets JAK1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | JAK1 fusion protein Ag25579 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Janus kinase 1 (a protein tyrosine kinase) |
| 别名 | EC:2.7.10.2, JAK 1, JAK-1, JAK1A, JAK1B |
| 计算分子量 | 1154 aa, 133 kDa |
| 观测分子量 | 125-133 kDa |
| GenBank蛋白编号 | BC132729 |
| 基因名称 | JAK1 |
| Gene ID (NCBI) | 3716 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P23458 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Janus kinase 1(JAK1), is a member of a class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. JAK1 is essential for signaling for certain type I and type II cytokines. JAK1 plays a critical role in initiating responses to multiple major cytokine receptor families. Expression of JAK1 in cancer cells enables individual cells to contract, potentially allowing them to escape their tumor and metastasize to other parts of the body.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for JAK1 antibody 27284-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |