验证数据展示
经过测试的应用
| Positive FC detected in | human PBMCs |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.2 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
FITC-65128 targets IgG2b Isotype Control in IF, FC applications and shows reactivity with n/a samples.
| 经测试应用 | FC Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | n/a |
| 免疫原 | N/A 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | immunoglobulin heavy chain 3 (serum IgG2b) |
| 别名 | Ighg2b, gamma2b, IgG2b, Igh-3, MPC-11 |
| 基因名称 | IgG2b Isotype Control |
| Gene ID (NCBI) | 16016 |
| RRID | AB_2883791 |
| 偶联类型 | FITC Plus Fluorescent Dye |
| 最大激发/发射波长 | 495 nm / 524 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P01867 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
The MPC-11 immunoglobulin is useful as an isotype-matched control. The MPC-11 immunoglobulin has an unknown binding specificity and is used as an isotype control for mouse IgG2b antibodies. This antibody has been quality-tested for flow cytometry as negative control.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for FITC Plus IgG2b Isotype Control antibody FITC-65128 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |