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验证数据展示

  • IF Staining of SH-SY5Y using CL488-15661

    IF Staining of SH-SY5Y using CL488-15661

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CoraLite® Plus 488 Islet 1 antibody (CL488-15661) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of SH-SY5Y using CL488-15661

    FC experiment of SH-SY5Y using CL488-15661

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Islet 1 (CL488-15661) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

CoraLite® Plus 488-conjugated Islet 1 Polyclonal antibody

Islet 1 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-15661

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

ISL1, Islet-1, ISLET1, ISL LIM homeobox 1, Isl 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inSH-SY5Y cells
For other applications, we recommend the unconjugated version of this antibody, 15661-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-15661 targets Islet 1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 Islet 1 fusion protein Ag8088 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ISL LIM homeobox 1
别名 ISL1, Islet-1, ISLET1, ISL LIM homeobox 1, Isl 1
计算分子量 39 kDa
观测分子量 39-45 kDa
GenBank蛋白编号BC031213
基因名称 Islet 1
Gene ID (NCBI) 3670
RRIDAB_3672636
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP61371
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ISL1 belongs to the LIM homeodomain transcription factor family that binds to the enhancer region of the INS gene. It plays a pivotal role in cardiogenesis, and lacking ISL1 expression results in growth arrest or displays profound defects in heart development, including atria, ventricle, and the inflow and outflow tracts.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Islet 1 antibody CL488-15661Download protocol
FC protocol for CL Plus 488 Islet 1 antibody CL488-15661Download protocol
Standard Protocols
Click here to view our Standard Protocols