验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells |
| Positive IHC detected in | mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
28378-1-AP targets Integrin beta-6 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | Human, Mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag29002 Product name: Recombinant human Integrin beta-6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 596-709 aa of BC121178 Sequence: DCVCGKCVCTNPGASGPTCERCPTCGDPCNSKRSCIECHLSAAGQAREECVDKCKLAGATISEEEDFSKDGSVSCSLQGENECLITFLITTDNEGKTIIHSINEKDCPKPPNIP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | integrin, beta 6 |
| 别名 | Integrin beta 6, Integrin beta-6, integrin, beta 6, ITGB6 |
| 计算分子量 | 788 aa, 86 kDa |
| 观测分子量 | 97 kDa |
| GenBank蛋白编号 | BC121178 |
| 基因名称 | Integrin beta 6 |
| Gene ID (NCBI) | 3694 |
| RRID | AB_2918157 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P18564 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ITGB6 belongs to the integrin beta chain family. ITGB6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of integrin alpha-V/beta-6 via clathrin-mediated endocytosis promotes carcinoma cell invasion.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Integrin beta-6 antibody 28378-1-AP | Download protocol |
| WB protocol for Integrin beta-6 antibody 28378-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Adv Biomimetic targeted self-adaptive nanodrug for inflammation optimization and AT2 cell modulation in precise ARDS therapy | ||
Chin Med J (Engl) Effect of TGFβ-ITGB6 feedback loop on liver metastasis in SMAD4 wild-type pancreatic cancer.
| ||
Respir Res Exosomal microRNAs of tuberculous pleural effusion orchestrating TGF-β signaling mediate pleural fibrosis. | ||
Transl Lung Cancer Res Exosomal ITGB6 from dormant lung adenocarcinoma cells activates cancer-associated fibroblasts by KLF10 positive feedback loop and the TGF-β pathway | ||
Front Oncol The association and clinicopathological significance of Integrin alphavbeta6 and Rac1 expression in gastric carcinoma | ||
PLoS One Odontogenesis-associated phosphoprotein (ODAPH) Promotes Ameloblast adhesion and alkaline phosphatase (ALP) expression via LAMC2/ ITGB6/TGF-β1 signaling pathway |
