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验证数据展示

  • WB analysis using 87465-1-RR

    WB analysis using 87465-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87465-1-RR (ITB5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 87465-1-RR

    IHC staining of mouse colon using 87465-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 87465-1-RR (Integrin beta 5 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of RAW 264.7 using 87465-1-RR

    IF Staining of RAW 264.7 using 87465-1-RR

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using Integrin beta 5 antibody (87465-1-RR, Clone: 252748B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity of 87465-1-RR

    Affinity of 87465-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87465-1-RR against Mouse Integrin beta 5 were performed. The affinity constant is 1.11 nM.

Integrin beta 5 Recombinant monoclonal antibody

Integrin beta 5 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 87465-1-RR

产品说明书

CloneNo. 252748B7

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Integrin beta-5, Itgb5

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inNeuro-2a cells, C6 cells, mouse lung tissue
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRAW 264.7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87465-1-RR targets Integrin beta 5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Eg10456

Product name: Recombinant mouse ITB5 protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-Twin-Strep-Tag

Domain: 10011459 of

Sequence: \

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 integrin beta 5
别名 Integrin beta-5, Itgb5
观测分子量100 kDa
基因名称 Itgb5
Gene ID (NCBI) 16419
RRIDAB_3745564
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Integrin beta-5 (ITGB5) is expected to be located in the plasma membrane and mitochondria, which is i ubiquitinated in placenta and gall bladder. ITGB5 is a member of integrins and was proved to be associated with pathological processes in several tumors (PMID: 34966851). It is reported that TGB5 is highly expressed in hepatocellular carcinoma (HCC), and miR-185 regulates the expression of β- catenin through the ITGB5-dependent manner and affects the proliferation and migration of HCC cells (PMID: 35223869).

实验方案

Product Specific Protocols
IF protocol for Integrin beta 5 antibody 87465-1-RRDownload protocol
IHC protocol for Integrin beta 5 antibody 87465-1-RRDownload protocol
WB protocol for Integrin beta 5 antibody 87465-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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