• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • FC experiment of human PBMCs using CL488-98171

    FC experiment of human PBMCs using CL488-98171

    1x10^6 human PBMCs were surface stained with CoraLite® Plus 647 Anti-Human CD3, and 5 ul CoraLite® Plus 488 Anti-Human Integrin alpha-5/CD49e Rabbit RecAb (CL488-98171, Clone: 241685D4) or CoraLite® Plus 488 Rabbit IgG Isotype Control RecAb (CL488-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human PBMCs using CL488-98171

    FC experiment of human PBMCs using CL488-98171

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human Integrin alpha-5/CD49e Rabbit RecAb (CL488-98171, Clone: 241685D4) (red) or CoraLite® Plus 488 Rabbit IgG Isotype Control RecAb (CL488-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

CoraLite® Plus 488 Anti-Human Integrin alpha-5/CD49e Rabbit Recombinant Antibody

Integrin alpha-5/CD49e Uni-rAb® Recombinant Antibody for FC
Cat No. CL488-98171

产品说明书

CloneNo. 241685D4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

Integrin Alpha-5, ITGA5, 241685D4, CD49 antigen-like family member E, CD49e

缓冲液配方:  PBS, Azide, BSA
PBS, Azide, BSA
偶联物:  CoraLite® Plus 488
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy ITGA5 Ready-To-Use IHC Kit. ITGA5 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 98171-1-PBS

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 5 ul per 10^6 cells in a 100 µl suspension
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-98171 targets Integrin alpha-5/CD49e in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原

Recombinant Protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
别名 Integrin Alpha-5, ITGA5, 241685D4, CD49 antigen-like family member E, CD49e
计算分子量 115 kDa
GenBank蛋白编号BC008786
基因名称 Integrin alpha 5
Gene ID (NCBI) 3678
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长491 nm / 516 nm
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP08648
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin alpha-5 (ITGA5, also known as CD49e), belongs to the integrin alpha chain family, primarily binds to Integrin beta 1 (ITGB1) to form an α5β1 heterodimer (PMID: 33711961). Integrin α5β1 is a specific receptor of fibronectin through its arginine-glycine-aspartic acid (RGD) binding site (PMID: 19608542). Integrin α5β1 plays roles in cell adhesion, migration and matrix formation, and has emerged as an essential mediator in many human carcinomas (PMID: 33408483).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 Integrin alpha-5/CD49e antibody CL488-98171Download protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat Able
正在加载，请稍候...